ISOLATION OF PRE-OSTEOCLASTS

本申请的目的，提交给NIDR小 赠款计划，是开发一种手段，隔离假定的预- 破骨细胞 三个必要的要素在此结合在一起， 使这一试点项目有可能取得成功建议：一， 大量前破骨细胞的来源已经确定; 二是一种无毒的荧光标记物， 可用;三，使用流式细胞仪， 可以将标记的细胞与其它细胞进行分选。 一旦 获得后，将通过形态学方法对细胞进行分型， 它们的生存能力、可培养性和融合形成 将评估多核破骨细胞。 的价值 奋进是双重的：首先，一个可靠的破骨细胞来源， 体外研究将是一种宝贵的研究工具。 二是 用于研究前体细胞融合机制的系统 将提供破骨细胞。 了解破骨细胞 由于破骨细胞活性过高， 是牙周病和其他疾病的重要方面 发生骨质流失的地方

The objectives of this application, submitted to the NIDR Small Grants Program, is to develop a means of isolating putative pre- osteoclasts. Three necessary elements come together in this proposal which make this pilot project likely to succeed: One, a source of large numbers of pre-osteoclasts has been identified; two, a non-toxic fluorescent marker to identify the cells is available; and three, access to a flow cytometer which will allow the sorting of the labeled cells from others is available. Once obtained, the cells will be typed by morphological methods, and their viability, culturability and capacity to fuse to form multinucleate osteoclasts will be assessed. The value of this endeavor is two-fold: First, a reliable source of osteoclasts for in vitro studies will be an invaluable research tool. Two, a system for investigating the mechanism of fusion of precursor cells into osteoclasts will be provided. Understanding osteoclast function has clinical relevance since excessive osteoclast activity is an important aspect of periodontal disease and other diseases where bone loss occurs.