ALCOHOL & EXPRESSION OF GABA/BZ RECEPTOR MESSINGER RNA

酒精

• 批准号: 3422027
• 负责人: ANDREJ ROTTER
• 金额: $ 7.35万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-28 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3422027
• 关键词: GABA receptor; alcoholic beverage consumption; autoradiography; central nervous system disorders; cow; gel electrophoresis; in situ hybridization; laboratory mouse; messenger RNA; molecular genetics; neuroanatomy; neurons; nucleic acid probes; radionuclides; receptor binding; receptor expression; receptor sensitivity; tissue /cell culture

Recent experimental studies have provided strong evidence that the gamma-aminobutyric acid/benzodiazepine (GABA/BZ) receptor mediates some of the effects of alcohol intoxication. The aim of this proposal is to study the effect of alcohol ingestion on the expression of GABA/BZ receptor subunit mRNAs in the mouse central nervous system. GABA/BZ receptor subunit mRNAs will be autoradiographically localized following Northern blot and in situ hybridization with subunit specific complementary [32p] and [35S]-oligonucleotide probes. These techniques will also be used to study the expression of GABA/BZ receptor subunit mRNA in granule cell cultures from developing normal mouse cerebella in the presence and absence of alcohol. The following experimental questions will be asked: What is the anatomical localization of subunit mRNAs coding for the alpha, beta and gamma subunits of the GABA/BZ receptor complex in adult murine central nervous system? How does the expression of subunit mRNAs coding for the alpha, beta and gamma subunits of the GABA/BZ receptor complex in the adult mouse brain change following chronic alcohol intoxication? Are there changes in the expression of MRNA coding for GABA/BZ receptor subunits in developing mouse brain in response to chronic maternal alcohol consumption? What is the difference in expression of GABA/BZ receptor subunit mRNAs in the CNS of ethanol-sensitive (LS) and ethanol-insensitive (SS) mice? How is the expression of GABA/BZ receptor subunit mRNAs in neuronal cultures of cerebellar granule cells affected by exposure to alcohol? The above studies will provide preliminary data of the effects of alcohol at the molecular genetic level in a specific neurotransmitter receptor system.

最近的实验研究提供了强有力的证据表明 γ-氨基丁酸/苯二氮卓 (GABA/BZ) 受体介导一些 酒精中毒的影响。本提案的目的是研究 酒精摄入对GABA/BZ受体表达的影响 小鼠中枢神经系统中的亚基 mRNA。 GABA/BZ 受体亚基 mRNA 将在 Northern blot 后进行放射自显影定位，并在 具有亚基特异性互补的原位杂交 [32p] 和 [35S]-寡核苷酸探针。这些技术也将用于研究 颗粒细胞培养物中 GABA/BZ 受体亚基 mRNA 的表达 在存在或不存在的情况下发育正常小鼠小脑 酒精。将提出以下实验问题：什么是 编码 α、β 和 mRNA 亚基的解剖定位 成年小鼠中枢 GABA/BZ 受体复合物的 γ 亚基 神经系统？编码亚基 mRNA 的表达如何 成人 GABA/BZ 受体复合物的 α、β 和 γ 亚基 慢性酒精中毒后小鼠大脑会发生变化吗？有吗 编码 GABA/BZ 受体亚基的 mRNA 表达变化 小鼠大脑的发育是为了应对母亲长期饮酒？ GABA/BZ受体亚基mRNA的表达有何差异 乙醇敏感（LS）和乙醇不敏感（SS）小鼠的中枢神经系统？怎么样 GABA/BZ 受体亚基 mRNA 在神经元培养物中的表达 接触酒精会影响小脑颗粒细胞吗？ 上述研究将提供酒精影响的初步数据 在特定神经递质受体的分子遗传水平 系统。