Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
批准号:
7732622
负责人:
William Paul
金额:
$33.9万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
Biological ProcessCD4 Positive T LymphocytesCandidate Disease GeneCell physiologyCellsClassClone CellsCytokine GeneDepthDevelopmentGene ProteinsGenesGeneticGenomeGoalsImmunoglobulinsInterleukin-13Interleukin-4LibrariesLymphocyteMediatingMethodsMicroarray AnalysisMusParticipantPathway interactionsPhenotypePhosphoric Monoester HydrolasesPhosphotransferasesPlayPopulationPreparationProcessProtein Tyrosine KinaseProtein Tyrosine PhosphataseProtocols documentationPurposeRNA InterferenceRangeRegulationRestRoleScreening procedureSet proteinSignal PathwaySignal TransductionSystemT-Cell ReceptorTechnologyTestingTransfectionbasecytokineinsightinterestprotein expressionresponsetool
中文摘要
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英文摘要
In order to gain a deeper insight into the genetic regulation of cytokine-determined and immunoglobulin/ T cell receptor based signaling in lymphocytes, efforts to use RNA interference (RNAi) technology as a screening tool have been undertaken. Selected libraries of shRNAs and siRNAs have been obtained. Introduction of siRNAs into resting CD4 T cells using Amaxa transfection technology has been achieved and, in test systems, impressive inhibition of expression and function of kinases such as Jak3 has been obtained. Currently, we have assembled a protein tyrosine kinase library and a protein tyrosine phosphatase library that will be tested for its capacity to inhibit or enhance the induction of Th2 phenotype by naive CD4 T cells. To this end, we have developed "recombineered" mice that faithfully expresses DS-Red as a surrogate for IL-13 and others that express Am-Cyan as a surrogate of IL-4 and destabilized DS-Red as a surrogate for IL-13. Thus, cells can be tested for induction or inhibition of surrogate expression and thus restimulation can be avoided. We anticipate both cloning cells that have been selected (i.e. either induced or suppressed, depending on the experimental protocol) and determining what shRNAs they have incorporated or using bulk induced or suppressed cells, carrying out microarray analysis of the shRNAs expressed by the bulk selected populations.
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Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
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批准号:8745429
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项目类别:
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资助金额:$10.21万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:7592157
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项目类别:
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资助金额:$162.54万
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财政年份:--
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负责人:William Paul
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依托单位:
Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
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批准号:7592323
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项目类别:
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资助金额:$38.58万
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财政年份:--
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负责人:William Paul
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依托单位:
Lymphocyte Dynamics
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批准号:8336169
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项目类别:
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资助金额:$129.17万
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财政年份:--
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负责人:William Paul
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依托单位:
Lymphocyte Dynamics
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批准号:7964486
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项目类别:
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资助金额:$118.49万
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财政年份:--
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负责人:William Paul
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依托单位:
Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
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批准号:8555902
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项目类别:
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资助金额:$29.07万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:7732461
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项目类别:
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资助金额:$149.35万
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财政年份:--
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负责人:William Paul
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依托单位:
INTERLEUKIN 4 (IL-4)
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批准号:6098948
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:William Paul
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依托单位:
Lymphocyte Dynamics
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批准号:8946366
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项目类别:
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资助金额:$99.94万
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财政年份:--
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负责人:William Paul
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依托单位:
Lymphocyte Dynamics
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批准号:8745403
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项目类别:
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资助金额:$91.86万
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财政年份:--
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负责人:William Paul
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依托单位:
Lymphocyte Dynamics
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批准号:8156948
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项目类别:
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资助金额:$141.57万
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财政年份:--
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负责人:William Paul
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依托单位:
Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
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批准号:8156977
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项目类别:
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资助金额:$46.79万
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财政年份:--
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负责人:William Paul
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依托单位:
Analyzing Cytokine- and TCR-Mediated Lymphocyte Responses by RNAi
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批准号:8336199
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项目类别:
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资助金额:$57.24万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:8946270
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项目类别:
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资助金额:$99.94万
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财政年份:--
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负责人:William Paul
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依托单位:
Eosinophilic Inflammatory Disease in Mice with Limited TCR Repertoire
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批准号:7964547
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项目类别:
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资助金额:$36.39万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:7964273
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项目类别:
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资助金额:$183.41万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:8555766
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项目类别:
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资助金额:$116.28万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:8336060
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项目类别:
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资助金额:$209.96万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:8745303
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项目类别:
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资助金额:$102.07万
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财政年份:--
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负责人:William Paul
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依托单位:
Interleukin 4
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批准号:8156845
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项目类别:
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资助金额:$221.41万
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财政年份:--
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负责人:William Paul
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依托单位: