Microscopy and Cellular Imaging Core

项目摘要/摘要 在第一和第二阶段，微生物致病和宿主炎症反应中心(CMPHIR) 建立和维护研究和技术进步核心，以填补 由UAMS医学院维持的机构核心中可用的资源。的主要关注点 核心是显微镜和细胞成像，目标是促进CMPHIR研究人员的能力 描述不同微生物病原体和特定宿主细胞之间的相互作用。购买的设备 为了支持这一努力，包括一台共焦显微镜、一台扫描电子显微镜和多种仪器 用于活细胞成像。日常业务将由主任Karen E.Beenken博士监督，他将 负责维护这些仪器的工作状态，培训CMPHIR调查人员，以及 其他UAMS调查人员，关于它的正确使用。为了促进自我可持续性和最大限度地提高可访问性，核心D 仪器将由用户自己操作，按使用费收费。这将允许用户访问 这些仪器在方便的时候，包括在非工作时间，并允许核心D恢复一个 相当大比例的成本，从而确保有能力升级和维护 在我们进入第三阶段及以后的过程中，我们可以根据需要选择核心。

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. A primary focus of the Core was on Microscopy and Cellular Imaging, the goal being to facilitate the ability of CMPHIR investigators to characterize the interaction between diverse microbial pathogens and specific host cells. Equipment purchased to support this effort included a confocal microscope, a scanning electron microscope, and multiple instruments for live cell imaging. Daily operations will be supervised by Dr. Karen E. Beenken, Director, who will be responsible for maintaining these instruments in working order and training CMPHIR investigators, as well as other UAMS investigators, on its proper use. To promote self-sustainability and maximize accessibility, Core D instruments will be operated by the users themselves on a fee-for-access basis. This will allow users access to these instruments at their convenience, including during non-working hours, and allow Core D to recover a significant proportion of the costs, thereby ensuring the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.