Microscopy and Cellular Imaging Core

显微镜和细胞成像核心

• 批准号: 10618386
• 负责人: Karen E. Beenken
• 金额: $ 22.8万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-05 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10618386
• 关键词: Address; Applications Grants; Cells; Centers of Research Excellence; Communicable Diseases; Core Facility; DNA sequencing; Data; Dedications; Development; Doctor of Philosophy; Electron Microscope; Elements; Ensure; Environment; Equipment; Fees; Flow Cytometry; Funding; Goals; Grant; Hour; Immune response; Infection; Inflammatory; Inflammatory Response; Institution; Laboratory Animal Medicine; Laboratory Personnel; Maintenance; Manuscripts; Medicine; Microscope; Modeling; Molecular Biology; Pathogenesis; Phase; Phase Transition; Play; Recovery; Research; Research Infrastructure; Research Personnel; Resources; Role; Scanning; Supervision; Technology; Therapeutic; Time; Training; base; biosafety level 3 facility; cellular imaging; college; cost; equipment acquisition; instrument; live cell imaging; member; microbial; microbial host; microscopic imaging; operation; pathogen; pathogenic microbe; programs; prophylactic; recruit; success; voucher

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. A primary focus of the Core was on Microscopy and Cellular Imaging, the goal being to facilitate the ability of CMPHIR investigators to characterize the interaction between diverse microbial pathogens and specific host cells. Equipment purchased to support this effort included a confocal microscope, a scanning electron microscope, and multiple instruments for live cell imaging. Daily operations will be supervised by Dr. Karen E. Beenken, Director, who will be responsible for maintaining these instruments in working order and training CMPHIR investigators, as well as other UAMS investigators, on its proper use. To promote self-sustainability and maximize accessibility, Core D instruments will be operated by the users themselves on a fee-for-access basis. This will allow users access to these instruments at their convenience, including during non-working hours, and allow Core D to recover a significant proportion of the costs, thereby ensuring the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.

项目总结/摘要 在第一阶段和第二阶段，微生物发病机制和宿主炎症反应中心（CMPHIR） 建立并维持了一个研究和技术进步核心，以填补实验空白， UAMS医学院维护的机构核心中可用的资源。一个主要的焦点 核心是显微镜和细胞成像，目标是促进CMPHIR研究人员的能力， 表征不同微生物病原体和特定宿主细胞之间的相互作用。购买的设备 为了支持这项工作，包括一个共聚焦显微镜，一个扫描电子显微镜，和多种仪器 用于活细胞成像日常操作将由Karen E博士监督。本肯主任谁将 负责维护这些仪器的工作状态，培训CMPHIR调查人员，以及 其他UAMS研究人员，对其正确使用。为了促进自我维持和最大限度地提高可获得性，核心D 仪器将由使用者自行操作，收费使用。这将允许用户访问 这些工具在他们方便的时候，包括在非工作时间，并允许核心D恢复一个 大部分的成本，从而确保升级和维护设备的能力， 在我们进入第三阶段及以后的过程中，