Development of a high precision and high order DNA counting platform

项目摘要/摘要 ENUMERIX已经开发出一种经济高效的方法来进行高精度的高阶DNA计数 称为直接计数聚合酶链式反应(DcPCR)。DcPCR使用旋转列方法生成数百万个 直接进入聚合酶链式反应试管，用于靶扩增，使用标准实验室离心机和 热循环仪，然后是一种超快速方法，直接从 同样的聚合酶链式反应试管。我们已经证明，我们的技术远远超过了数字技术的精度 聚合酶链式反应，可以在类似于定量聚合酶链式反应和下一代的DNA计数范围内进行 测序，这使它成为一个合适的下一代DNA计数平台。对于此建议的阶段 II项目我们计划进一步开发我们的dcPCR平台，目标是将技术分发到 最终用户取代定量聚合酶链式反应和数字聚合酶链式反应。

PROJECT SUMMARY/ABSTRACT Enumerix has developed a cost-effective method to perform high order DNA counting at high precision called direct count PCR (dcPCR). dcPCR uses a spin column method that generates millions of partitions directly into PCR tubes for target amplification, using standard laboratory centrifuge and thermal cyclers, followed by an ultra-rapid method to count up to 1 million copies of DNA directly from the same PCR tube. We have demonstrated that our technology far exceeds the precision of digital PCR, and can perform at a DNA counting range akin to quantitative PCR and next generation sequencing, which makes it a suitable next-generation DNA counting platform. For this proposed Phase II project we plan to further develop our dcPCR platform with the goal to distribute the technology to end-users to replace quantitative PCR and digital PCR.