Development of a high precision and high order DNA counting platform

项目概要/摘要 Enumerix开发了一种具有成本效益的方法，可以高精度地进行高阶DNA计数 称为直接计数PCR（DCPCR）。dcPCR使用旋转柱方法， 使用标准实验室离心机，直接将分区放入PCR管中进行靶扩增， 热循环仪，然后是一种超快速的方法，直接从DNA中计数高达100万个拷贝， 同一个PCR管我们已经证明，我们的技术远远超过数字的精度 PCR，并且可以在类似于定量PCR和下一代PCR的DNA计数范围内进行 测序，这使其成为合适的下一代DNA计数平台。对于本拟定阶段 II项目，我们计划进一步开发我们的dcPCR平台，目标是将该技术分发给 最终用户取代定量PCR和数字PCR。

PROJECT SUMMARY/ABSTRACT Enumerix has developed a cost-effective method to perform high order DNA counting at high precision called direct count PCR (dcPCR). dcPCR uses a spin column method that generates millions of partitions directly into PCR tubes for target amplification, using standard laboratory centrifuge and thermal cyclers, followed by an ultra-rapid method to count up to 1 million copies of DNA directly from the same PCR tube. We have demonstrated that our technology far exceeds the precision of digital PCR, and can perform at a DNA counting range akin to quantitative PCR and next generation sequencing, which makes it a suitable next-generation DNA counting platform. For this proposed Phase II project we plan to further develop our dcPCR platform with the goal to distribute the technology to end-users to replace quantitative PCR and digital PCR.