Mutagenesis and Phenotyping Core

该核心是我们的P01应用程序的核心元素。它负责产生， 确定并保存这三个项目将使用的小鼠不孕模型，以及 将向研究界提供。核心的基石是生成大型 对突变小鼠的数量进行全基因组筛查，寻找导致小鼠不育的突变。这个 第二个主要组成部分是不育突变的表型，并对它们进行可能的分类 随后由三个项目之一进行分析。突变将得到基因验证，然后要么 通过一个或多个项目进行扩展以进行进一步的详细研究，或进行冷冻保存并提供给 通过个人联系人和计划项目网站访问整个社区。下面是具体的角色 更详细地概述了核心的部分。

This core is the central element of our P01 application. It is responsible for generating, identifying, and preserving the mouse infertility models that will be used by the three Projects, and which will be made available to the research community. The cornerstone of the Core is to generate large numbers of mutant mice and conduct a genome-wide screen for mutations causing sterility in mice. The second major component is phenotyping of infertility mutants, and to classify them for possible subsequent analysis by one of the three Projects. Mutations will be validated genetically, and then either expanded for further detailed studies by one or more of the Projects, or cryopreserved and offered to the community at large through personal contacts and the Program Project Website. Below, the specific roles of the core are outlined in more detail.