Mutagenesis and Phenotyping Core

诱变和表型核心

• 批准号: 7952300
• 负责人: MARY ANN HANDEL
• 金额: $ 16.7万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7952300
• 关键词: Affect; Animals; Breeding; Chemicals; Classification; Collection; Communities; Databases; Development; Elements; Ethylnitrosourea; Etiology; Frequencies; Genetic Materials; Genomics; Germ Cells; Goals; Human; Infertility; Modeling; Mus; Mutagenesis; Mutant Strains Mice; Mutation; Phenotype; Procedures; Reagent; Reproductive Biology; Research; Resource Allocation; Resources; Role; Scheme; Sterility; Technology; Work; embryonic stem cell; genome wide association study; mutant; novel; programs; reproductive; web interface; web site

This core is the central element of our P01 application. It is responsible for generating, identifying, and preserving the mouse infertility models that will be used by the three Projects, and which will be made available to the research community. The cornerstone of the Core is to generate large numbers of mutant mice and conduct a genome-wide screen for mutations causing sterility in mice. The second major component is phenotyping of infertility mutants, and to classify them for possible subsequent analysis by one of the three Projects. Mutations will be validated genetically, and then either expanded for further detailed studies by one or more of the Projects, or cryopreserved and offered to the community at large through personal contacts and the Program Project Website. Below, the specific roles of the core are outlined in more detail.

这个核心是我们P01应用程序的核心元素。它负责生成， 确定和保存三个项目将使用的小鼠不育模型， 将提供给研究界。核心的基石是产生大量的 突变小鼠的数量，并进行全基因组筛选导致小鼠不育的突变。的 第二个主要组成部分是不育突变体的表型，并将其分类为可能的 三个项目之一的后续分析。突变将通过基因验证，然后 由一个或多个项目进行进一步的详细研究，或冷冻保存并提供给 通过个人联系人和计划项目网站向整个社区提供信息。下面是具体角色 的核心是更详细地概述。