NTHi Immunity in Young Children

幼儿的 NTHi 免疫力

• 批准号: 7850299
• 负责人: MICHAEL E PICHICHERO
• 金额: $ 30.98万
• 依托单位: ROCHESTER GENERAL HOSPITAL (NY)
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-17 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7850299
• 关键词: Acoustics; Acute; Age; Antibodies; Antibody Formation; Antibody Repertoire; Avidity; Bacterial Adhesins; Bacterial Infections; Blood; Child; Childhood; Clinical; Clinical Trials; Collaborations; Developed Countries; Developing Countries; Diagnostic; Disease; Educational workshop; Elements; Event; Future; Goals; Haemophilus influenzae ompP2 protein; Health; Heterogeneity; Human; Immune; Immune response; Immunity; Intervention; Knowledge; Lead; Liquid substance; Modeling; Moraxella (Branhamella) catarrhalis; Morbidity - disease rate; Nasal Lavage Fluid; National Institute of Allergy and Infectious Disease; Nontypable Haemophilus influenza; Organism; Otitis; Otitis Media; Otoscopy; Pathogenesis; Prevention; Process; Proteins; Public Health; Recording of previous events; Recruitment Activity; Research; Research Personnel; Risk; Role; Sampling; Serum; Site; Streptococcus pneumoniae; Technology; Time; Tympanometry; Tympanostomy; United States National Institutes of Health; Vaccines; cohort; diagnostic accuracy; experience; insight; middle ear; prevent; repository; response; suburb; time interval; vaccine candidate; vaccine development

DESCRIPTION (provided by applicant): Nontypable Haemophilus influenzae (NTHi) is one of the major etiological agents of acute otitis media (AOM) which has very high morbidity among children in both developing and developed countries. The long term goal of this research is to better understand NTHi pathogenesis and human immune responses following nasopharyngeal (NP) colonization and AOM, in order to develop strategies for intervention in the disease. We will prospectively collect NP, middle ear fluids (MEF), and sera from young children age 2 to 24 months, who are otitis prone, have infrequent otitis, or remain otitis free. We propose to evaluate the role of specific immune responses to NTHi outer membrane proteins P2, P5, P6, and OMP26 in clearance of NP colonization by comparing children who clear colonization with those who experience prolonged colonization. In addition, we will establish a repository of prospectively collected mucosal and serum samples for other researchers in otitis media. The specific aims of this research are: Specific Aim 1: To prospectively collect NP secretions, MEF, and sera from young children who are otitis prone, have infrequent otitis, or remain otitis free. Specific Aim 2: To evaluate the role of specific immune responses to NTHi P2, P5, P6, and OMP26 in clearance of AOM by comparing children with different AOM experiences and in clearance of NP colonization by comparing children with different colonization experiences. Specific Aim 3: To establish a repository of prospectively collected mucosal and serum samples for future research in otitis media. Relevance to Public Health: This project is specifically responsive to research needs identified at an NIAID and NIDCD-organized workshop held at the NIH on 8-9 June 2004 on "Vaccines for Otitis Media: Proposals for Overcoming Obstacles to Progress." The results gained from these studies will provide insight to the immune mechanisms involved in the pathogenesis of otitis media aid in the development of a vaccine for otits media.

描述（由申请方提供）：非典型流感嗜血杆菌（NTHi）是急性中耳炎（AOM）的主要病原体之一，在发展中国家和发达国家的儿童中，AOM的发病率非常高。本研究的长期目标是更好地了解NTHi发病机制和鼻咽（NP）定植和AOM后的人体免疫反应，以便制定干预该疾病的策略。我们将前瞻性地收集2至24个月大的幼儿的NP、中耳液（MEF）和血清，这些幼儿是易患耳炎的、不常患耳炎的或保持无耳炎的。我们建议通过比较清除定植的儿童与经历长期定植的儿童来评估对NTHi外膜蛋白P2、P5、P6和OMP26的特异性免疫应答在清除NP定植中的作用。此外，我们将为中耳炎的其他研究人员建立一个前瞻性收集的粘膜和血清样本库。本研究的具体目的是：具体目的1：前瞻性地收集NP分泌物，MEF，和血清的幼儿谁是中耳炎易感，有罕见的中耳炎，或保持中耳炎免费。具体目标二：通过比较具有不同AOM经历的儿童来评估对NTHi P2、P5、P6和OMP 26的特异性免疫应答在AOM清除中的作用，以及通过比较具有不同定植经历的儿童来评估对NP定植的清除中的作用。具体目标3：建立前瞻性收集的粘膜和血清样本库，用于中耳炎的未来研究。与公共卫生的相关性：该项目专门响应NIAID和NIDCD于2004年6月8日至9日在NIH举办的关于“中耳炎疫苗：克服进展障碍的建议”的研讨会上确定的研究需求。“从这些研究中获得的结果将为中耳炎发病机制中涉及的免疫机制提供见解，有助于开发中耳炎疫苗。