Core B - Technical

核心 B - 技术

• 批准号: 8015759
• 负责人: LAWRENCE D LONGO
• 金额: $ 34.31万
• 依托单位: LOMA LINDA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8015759
• 关键词: Adrenal Glands; Altitude; Animals; Budgets; Cerebrum; Collaborations; Confocal Microscopy; Core Facility; Data; Equipment; Fetus; Hypoxemia; Image; Individual; Laboratories; Laboratory Animals; Operative Surgical Procedures; Physiological; Primates; Principal Investigator; Pulmonary artery structure; Radioactivity; Research; Research Personnel; Research Support; Sheep; System; Time; Tissues; Western Blotting; animal facility; animal tissue; base; blood gas analyzer; data acquisition; member; operation; pregnant; research study

A. Introduction. Pregnant sheep will continue to be used for these studies, as they are readily available, are relatively inexpensive (as compared with primates), the fetuses are large enough so that adequate amounts of the several tissues can be studied (cerebral, uterine, and pulmonary arteries, adrenal glands, ef cetera), and we and others have amassed a considerable body of physiologic data on this species. Essentially all of the individual projects in this proposal will use pregnant or nonpregnant sheep which have been acclimatized to high altitude, long-term hypoxemia, or normoxic controls. At the time of sacrifice, tissues from these animals will be partitioned among the several investigators and projects of this proposal, as well as several other investigators. Thus, this Technical Core includes the animals for these studies. In addition, it includes the expense for several major items of equipment required for most of these experiments. The experimental animal and equipment Technical Core will be utilized for essentially every project in common by all members of the Center. Such a Technical Core optimizes the efficient operation of the laboratories, the experiments per se, and the data acquisition and analysis. The essential features of the experimental animal procurement and laboratory are detailed below. Following a general description of our laboratories, equipment, and laboratory animal facilities, the Technical Core will be considered from several aspects: Experimental Animals, White Mountain Research Station, LLU Animal Facilities, Laboratory Core Facilities, and Budget. Drs. Longo and Ducsay will devote 10% of their time to administer the Technical Core. The principal investigators will continue to share major items of equipment including: imaging systems, ultracentrifuges, centrifuges, radioactivity counters, recorders, blood-gas analyzers, analyzers for Western immunoblot, realtime PCR, confocal microscopy, freezers, and other items. In addition, we will continue to share the experimental animals, and surgical operating theater. This fiscally efficient arrangement, together with the scientific collaboration among the investigators js one of the reasons that broad based research support is sought.

A.导论.妊娠绵羊将继续用于这些研究，因为它们容易获得，相对便宜（与灵长类动物相比），胎儿足够大，可以研究足够数量的几种组织（脑动脉、子宫动脉、肺动脉、肾上腺等），我们和其他人已经积累了大量关于该物种的生理数据。基本上，本提案中的所有单个项目都将使用已适应高海拔、长期低氧血症或常氧对照的妊娠或非妊娠绵羊。处死时，将这些动物的组织分配给本提案的几名研究者和项目以及其他几名研究者。因此，本技术核心包括这些研究的动物。此外，它还包括大多数这些实验所需的几个主要设备项目的费用。实验动物和设备技术核心将用于中心所有成员共同的基本每个项目。这样的技术核心优化了实验室、实验本身以及数据采集和分析的有效操作。实验动物采购和实验室的基本特征详述如下。在对我们的实验室、设备和实验动物设施进行一般描述后，将从几个方面考虑技术核心：实验动物、白色山研究站、LLU动物设施、实验室核心设施和预算。 Drs. Longo和Ducsay将投入10%的时间来管理技术核心。校长 研究人员将继续共享主要设备，包括：成像系统、超浓缩机、离心机、放射性计数器、记录仪、血气分析仪、Western免疫印迹分析仪、实时PCR、共聚焦显微镜、冷冻机和其他设备。此外，我们将继续分享实验动物和手术室。这种高效率的安排，加上研究人员之间的科学合作，是寻求广泛的研究支持的原因之一。