Genome-Wide Regulation of the TATA Binding Protein

TATA 结合蛋白的全基因组调控

• 批准号: 7899663
• 负责人: B FRANKLIN PUGH
• 金额: $ 43.21万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-14 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7899663
• 关键词: Acetylation; Acetyltransferase; Address; Binding; Biochemical; Biochemical Genetics; Biological; Biological Assay; Biological Models; Bromodomain; Chromatin; Complex; Couples; Data Analyses; Dependency; Disease; Event; Fibrinogen; Funding; Gene Expression; Gene Expression Regulation; Genes; Genetic Transcription; Genomics; Health; Heat-Shock Response; Histone Acetylation; Histones; Holoenzymes; Housekeeping Gene; Human; In Vitro; Individual; Instruction; Lead; Lysine; Mediator of activation protein; Molecular; Molecular Genetics; Monitor; Motion; Mutate; Nucleosomes; Pathway interactions; Phase; Positioning Attribute; Process; Protein Binding; RNA Polymerase II; Reading; Recruitment Activity; Regulation; Research; Resources; SAGA; Saccharomyces; Saccharomyces cerevisiae; Saccharomycetales; Stress; System; TATA Box; TATA-Box Binding Protein; Tail; Techniques; Testing; Tin; Work; abstracting; base; chromatin immunoprecipitation; chromatin remodeling; gene function; genetic regulatory protein; genome-wide; in vivo; mutant; promoter; public health relevance; research study; transcription factor

DESCRIPTION (provided by applicant): Project Summary/Abstract Human health is highly dependent upon proper gene expression. Understanding how genes are regulated is critical in our understanding how mis- regulation leads to diseases. One major point where genes are regulated is during the assembly of the transcription machinery. This project is focused on understanding the molecular events that govern the assembly of the transcription machinery at promoters. The model system is the budding yeast Saccharomyces cerevisiae, because of its experimental tractability and the mechanistic similarities to assembly of the transcription machinery in humans. The project will assess the extent to which individual transcription regulators are dependent upon other transcriptional regulator for binding to the promoters of all Saccharomyces genes. The project will examine in greater mechanistic details portions of the assembly process that involve recruitment of the TATA binding protein (TBP) to promoters, as TBP is a central linchpin in the assembly process. In particular, the interplay between chromatin and two major TBP delivery complexes (TFIID and SAGA) will be investigated. PUBLIC HEALTH RELEVANCE: Project Narrative Relevance Human health is highly dependent upon the proper functioning of our genes (biological instructions). This project will contribute to a greater understanding of how genes function by determining how the gene regulatory proteins work together to read the instructions on a global scale.

描述（由申请人提供）： 人类健康高度依赖于适当的基因表达。了解基因是如何被调控的，对于我们理解错误调控是如何导致疾病的至关重要。基因调控的一个主要点是在转录机器的组装过程中。这个项目的重点是了解在启动子的转录机制的组装管理的分子事件。该模型系统是芽殖酵母酿酒酵母，因为它的实验易处理性和机制的相似性组装的转录机器在人类。该项目将评估在何种程度上个别转录调节依赖于其他转录调节结合到所有酵母基因的启动子。该项目将在更大的机械细节部分的组装过程中，涉及招聘的TATA结合蛋白（TBP）的启动子，因为TBP是在组装过程中的核心关键。特别是，染色质和两个主要的TBP传递复合物（TFIID和佐贺）之间的相互作用将进行研究。公共卫生相关性：人类健康高度依赖于我们基因的正常功能（生物指令）。该项目将通过确定基因调控蛋白如何共同工作以在全球范围内阅读指令来更好地理解基因的功能。