Genome-Wide Regulation of the TATA Binding Protein

描述(由申请人提供)： 项目摘要/摘要人类的健康高度依赖于适当的基因表达。了解基因是如何调控的，对于我们理解错误调控如何导致疾病至关重要。基因调控的一个主要环节是转录机制的组装过程。这个项目的重点是了解控制转录机制在启动子上组装的分子事件。该模型系统是萌芽酵母酿酒酵母，因为它的实验可操作性和与人类转录机器组装的机制相似。该项目将评估单个转录调节因子依赖于其他转录调节因子与所有酵母基因的启动子结合的程度。该项目将更详细地研究组装过程中涉及将TATA结合蛋白(TBP)招募到启动子的部分，因为TBP是组装过程中的中心关键。特别是，染色质和两个主要的TBP递送复合体(TFIID和SAGA)之间的相互作用将被研究。公共卫生相关性：项目叙事相关性人类健康高度依赖于我们基因的正常功能(生物学说明)。该项目将通过确定基因调控蛋白如何在全球范围内协同工作来阅读指令，从而有助于更好地理解基因的功能。

DESCRIPTION (provided by applicant): Project Summary/Abstract Human health is highly dependent upon proper gene expression. Understanding how genes are regulated is critical in our understanding how mis- regulation leads to diseases. One major point where genes are regulated is during the assembly of the transcription machinery. This project is focused on understanding the molecular events that govern the assembly of the transcription machinery at promoters. The model system is the budding yeast Saccharomyces cerevisiae, because of its experimental tractability and the mechanistic similarities to assembly of the transcription machinery in humans. The project will assess the extent to which individual transcription regulators are dependent upon other transcriptional regulator for binding to the promoters of all Saccharomyces genes. The project will examine in greater mechanistic details portions of the assembly process that involve recruitment of the TATA binding protein (TBP) to promoters, as TBP is a central linchpin in the assembly process. In particular, the interplay between chromatin and two major TBP delivery complexes (TFIID and SAGA) will be investigated. PUBLIC HEALTH RELEVANCE: Project Narrative Relevance Human health is highly dependent upon the proper functioning of our genes (biological instructions). This project will contribute to a greater understanding of how genes function by determining how the gene regulatory proteins work together to read the instructions on a global scale.