Structural Biology Center for HIV/HOST Interactions in Trafficking and Assembly

我们将建立一个综合性中心，研究艾滋病毒/宿主相互作用的结构生物学， 病毒贩运和组装。我们的工作将集中在四个领域：1）病毒RNA和Gag贩运，2） 病毒出芽，3）病毒结构，和4）早期事件和TRIMSa限制。我们的研究旨在 明确Rev依赖的核输出的分子基础，揭示宿主/病毒复合物的结构 以及促进病毒出芽的酶，并在体内重建未成熟和成熟的HIV-1病毒体。 前所未有的细节。合作互动将扩大我们的研究，包括结构研究， TRIMSa限制、HIV-1 Nef的感染性增强以及一系列宿主因素参与了HIV-1 Nef的表达。 病毒复制的早期阶段我们还将开发和应用新的计算和实验 分析宿主/病毒复合体的方法，成像它们如何在细胞中运输，研究它们如何发挥作用 作为分子机器，抑制它们的功能。这项工作将为新的抗病毒药物奠定基础。 这也将有助于将艾滋病毒发展成为一个无与伦比的模型系统，用于研究人类病毒是如何 与宿主细胞相互作用

We will establish a comprehensive Center to study the structural biology of HIV/Host interactions involved in viral trafficking and assembly. Our work will be focused in four areas: 1) Viral RNA and Gag Trafficking, 2) Virus Budding, 3) Virus Structure, and 4) Early Events and TRIMSa Restriction. Our studies are designed to define the molecular basis for Rev-dependent nuclear export, reveal the structures of host/virus complexes and enzymes that facilitate virus budding, and reconstruct the immature and mature HIV-1 virions in unprecedented detail. Collaborative interactions will extend our research to include structural studies of TRIMSa restriction, the infectivity enhancement of HIV-1 Nef, and a series of host factors involved in the early stages of viral replication. We will also develop and apply new computational and experimental methods for analyzing host/virus complexes, imaging how they traffic in the cell, studying how they function as molecular machines, and inhibiting their functions. This work will lay the groundwork for new antiviral strategies and will also help develop HIV into an unparalleled model system for studying how a human virus interacts with its cellular host.