Metabolism

新陈代谢中心的目标是为PPG调查人员提供科学和技术支持 支持对所有四个国家的血液和心肌组织样本进行生化分析 PPG中的项目。核心将对血液和组织进行代谢和同位素示踪分析 这些狗在项目1、2和3中进行了研究，并将在小鼠身上监督双标记水的实验 核心还将评估选定的蛋白质的表达和活性，测量 关键调节代谢物(如三磷酸腺苷、乳酸等)的含量，测量选定的 基因和血浆激素水平。项目1的炎性细胞因子的测量将是 由核心执行。这些测量所需的所有专业知识和设备都已到位。

The objective of the Metabolism core is to provide the to PPG investigators the scientific and technical support for the execution of biochemical analyses of blood and myocardial tissue samples from all four Projects in the PPG. The Core will perform metabolic and isotopic tracer analysis of blood and tissue from the dogs studied in Projects 1, 2 and 3, and will oversee the experiments with doubly labeled water in mice and dogs in Project 1. The Core will also assess the expression and activity of selected proteins, measure the content of key regulatory metabolites (e.g ATP, lactate, etc), measure mRNA expression for selected genes, and plasma hormone levels. The measurement of Inflamatory cytokines for Project 1 will be performed by the Core. All of the expertise and equipment necessary for these measurements is in place.