Physiological and Molecular Imaging

生理和分子成像

• 批准号: 7753056
• 负责人: Tanya N Mayadas
• 金额: $ 18.94万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7753056
• 关键词: Affect; Animals; Antibodies; Arts; Binding; Biology; Blood Vessels; Blood flow; Bone Marrow; Cell Communication; Cells; Computer-Assisted Image Analysis; Data Analyses; Development; Dorsal; Ear; Endothelium; Equipment; Fluorescence; Functional disorder; Goals; Hour; Human Resources; Image; Immersion Investigative Technique; In Vitro; Individual; Instruction; Knowledge; Label; Laboratories; Lamina Propria; Life; Mesentery; Microcirculation; Microscope; Modeling; Muscle; Operative Surgical Procedures; Organ; Peripheral; Physiological; Preparation; Productivity; Proteins; Research; Research Personnel; Resources; Saline; Services; Skin; Structure of aggregated lymphoid follicle of small intestine; Structure of jugular vein; System; Technical Expertise; Techniques; Time; Training; Work; base; blood perfusion; cellular imaging; cremaster muscle; data acquisition; femoral artery; in vivo; instrument; intravenous injection; intravital microscopy; lens; lymph nodes; molecular imaging; programs; tissue preparation; training project

The goal of this Core is to provide "real fime" physiological and molecular imaging support for various experimental applicafions in this Program. The Core will provide equipment and expertise for the qualitative and quantitafive analysis of cellular and molecular interactions at two different levels: cell-cell and cellmolecular interactions in vitro, and cell-cell interactions in vivo in the context of the vessel wall. The in vitro component of this Core will give Pis access to live cell imaging capabilifies, and computer assisted image analysis, of unlabeled cells, or cells labeled with fluorescently tagged antibodies or transduced to express GFP-tagged protein. This component is referred to as the Live cell fluorescence Microscopv facility. For in vivo studies, the established Intravital Microscopv (IVM) facility in The Center for Excellence in Vascular Biology will make available state-of the art capabilities for real-time imaging ofthe microcirculation in anesthetized animals. Thus through this Core, Program investigators will leverage resources that would be too expensive for single laboratories to purchase and maintain. Importanfiy, the Core will provide expertise and technical assistance to increase the productivity of individual projects as well as increase the capabilities of the Pis to accomplish the experimental aims of each project.

该核心的目标是提供“真实的fime”生理和分子成像支持， 在这个项目中的实验应用。核心将提供设备和专业知识， 在细胞-细胞和细胞分子两个不同水平上定量分析细胞和分子的相互作用 在体外的相互作用和在血管壁的情况下的体内的细胞-细胞相互作用。体外 该核心的组成部分将使PI获得活细胞成像能力， 分析未标记的细胞，或用荧光标记的抗体标记的细胞或转导以表达 GFP标记蛋白。该组件被称为活细胞荧光显微镜设施。用于体内 研究中，血管生物学卓越中心已建立的活体显微镜（IVM）设施将 使麻醉动物的微循环实时成像技术达到最先进水平。 因此，通过这个核心，项目调查人员将利用资源，这对单一的项目来说太昂贵了。 实验室购买和维护。重要的是，核心将提供专门知识和技术援助， 提高单个项目的生产力，并提高PI完成 每个项目的实验目标。