ROLE OF IGFBP-2 FRAGMENTS IN DEVEL OF ANDROGEN INSENS PROSTATE CANCER

IGFBP-2 片段在无雄激素前列腺癌发展中的作用

• 批准号: 7960174
• 负责人: Robert Walton Sikes
• 金额: $ 5.46万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960174
• 关键词: Androgens; Chinese Hamster Ovary Cell; Computer Retrieval of Information on Scientific Projects Database; Delaware; Development; Doctor of Philosophy; Funding; Grant; Institution; Malignant neoplasm of prostate; Open Reading Frames; Peptides; Proteolysis; Recombinants; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; androgen independent prostate cancer; design; expression vector; human IGFBP2 protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Robert A. Sikes, Ph.D. Role of IGFBP-2 Fragments in Development of Androgen Insensitive Prostate Cancer This project was designed to 1) Isolate and obtain sequence information from IGFBP-2 fragments, 2) to clone the IGFBP-2 open reading frame into an expression vector where the ends are modified by adding small peptide tags, and 3) to introduce and express these constructs in CHO cells to obtain tagged recombinant IGFBP-2 to study proteolysis.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Robert A. Sikes博士 IGFBP-2片段在雄激素不敏感前列腺癌发生中的作用 本项目旨在1）从IGFBP-2片段中分离并获得序列信息，2）将IGFBP-2开放阅读框架克隆到表达载体中，并通过添加小肽标签修饰末端，3）将这些构建体引入CHO细胞中并表达，以获得带标签的重组IGFBP-2，以研究蛋白水解。