AMIDE-TO-ESTER MUTATIONS IN INVESTIGATING FOLDING OF HELICAL PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 骨架-骨架氢键（H-键）是天然蛋白质结构的常见且明显重要的特征，但它们在折叠过渡态结构中的作用尚不清楚。一种可能性是H-键是折叠期间天然侧链接触形成的结果。另一种可能性是，它们通过提供一个框架来引导折叠流向原生状态，从而发挥更决定性的作用。为了更好地理解骨架-骨架氢键在蛋白质折叠中的动力学影响，我们研究了螺旋氢键在绒毛头部亚结构域（HP-35）折叠动力学中的作用，使用Phi值分析方法结合酰胺-酯（A-to-E）骨架诱变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Backbone-backbone hydrogen bonds (H-bond) are common and apparently important features of native protein structures, yet their role in folding transition state structure remains unclear. One possibility is that H-bonds result as a consequence of the formation of native sidechain contacts during folding. Another possibility is that they play a more deciding role by providing a framework that directs the flow of folding toward the native state. To provide a better understanding of the kinetic influence of backbone-backbone H-bonds in protein folding, we investigate the role of helical H-bonding in the folding kinetics of the villin headpiece subdomain (HP-35) using the Phi-value analysis method in conjunction with amide-to-ester (A-to-E) backbone mutagenesis.