Metagenomic Analysis of Ocular Surface Microbiome

眼表面微生物组的宏基因组分析

• 批准号: 7991283
• 负责人: VALERY I SHESTOPALOV
• 金额: $ 22.8万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7991283
• 关键词: Address; Affect; Age; Arts; Bacterial Genes; Bioinformatics; Biological Assay; Cataloging; Catalogs; Clinical; Communities; Conjunctival Epithelium; Contact Lenses; Cornea; DNA; DNA Sequence; Data; Databases; Diagnostic; Disease; Ecology; Endophthalmitis; Ethnic Origin; Eye; Eye Infections; Foundations; Gender; Genes; Genomics; Goals; Health; Human; Indigenous; Individual; Inflammation; Institutes; Keratitis; Knowledge; Laboratories; Metabolic; Metagenomics; Microbe; Microbiology; Monitor; Pathway interactions; Phase; Phylogeny; Play; Population; Recruitment Activity; Research; Research Personnel; Ribosomal RNA; Role; Sampling; Shotgun Sequencing; Structure; Surveys; Techniques; Technology; Testing; Therapeutic; Time; Tissues; Trachoma; Virulence; Vision; base; conjunctiva; corneal epithelium; design; effective therapy; eye dryness; human DNA; microbial; microbial community; microbiome; microorganism; multidisciplinary; next generation; novel; novel diagnostics; ocular surface; pathogen; phase 2 study; public health relevance; tool

DESCRIPTION (provided by applicant): Microbial invasion into the ocular surface (OS) compromises corneal clarity and causes inflammation in blinding conditions like keratitis, endoophthalmitis and trachoma. However, in many cases the pathogen(s) remains unidentified because it fails to grow in culture. In this project we will utilize latest DNA sequencing technology to overcome this limitation. Our ultimate goal is to investigate the role of commensal microbial population of the human OS in health and disease. Our approach is to use the "culture-independent" technology that detects and assigns non-human DNA to taxonomically distinct microbial species. The same technology in combination with bioinformatics will be utilized to annotate microbial genes to functional pathways, in order to determine potential activity of microbial population on the OS. We will analyze samples of healthy conjunctiva and corneas to examine differences in microbial communities ("microbiome") in different compartments of ocular surface. We put forth a coordinated effort of multidisciplinary team at Bascom Palmer Eye Institute and microbial genomics experts at the Argonne National Laboratory (Argonne, IL) to investigate, for the first time, the OS microbiome. The new knowledge acquired in this project is expected to revolutionize the understanding of ocular ocular microbial ecology, and facilitate designing of novel diagnostic tools. Successful completion of this project will advance the field towards testing the therapeutic potential of manipulation the OS microbiome. PUBLIC HEALTH RELEVANCE: The ultimate goal of this new project is recruit new sequencing technology to detect, identify and catalogue all microorganisms that populate human ocular surface, and can potentially affect vision. We will undertake the first comprehensive characterization of microbial diversity of healthy ocular surface that will allow us to monitor bacterial diversity in healthy and diseased eyes. Successful completion of this project will bring to a new level both diagnostics and understanding of ocular infections. PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page Continuation Format Page

描述(申请人提供)：微生物侵入眼表(OS)会损害角膜的透明度，并在致盲条件下引起炎症，如角膜炎、眼内炎和沙眼。然而，在许多情况下，病原体(S)仍未确定，因为它无法在培养中生长。在这个项目中，我们将利用最新的DNA测序技术来克服这一限制。我们的最终目标是调查人类OS的共生微生物种群在健康和疾病中的作用。我们的方法是使用“独立于培养”的技术来检测非人类DNA，并将其分配给分类上不同的微生物物种。将利用相同的技术与生物信息学相结合，将微生物基因注释为功能途径，以确定微生物种群在OS上的潜在活动。我们将分析健康的结膜和角膜的样本，以检查眼表不同隔室中微生物群落(微生物群)的差异。我们由巴斯科姆·帕尔默眼科研究所的多学科团队和伊利诺伊州阿贡国家实验室(Argonne，IL)的微生物基因组学专家共同努力，首次研究OS微生物群。在这个项目中获得的新知识有望彻底改变对眼部微生物生态学的理解，并促进新诊断工具的设计。该项目的成功完成将推动该领域朝着测试操纵OS微生物组的治疗潜力的方向发展。 与公共卫生相关：这个新项目的最终目标是招募新的测序技术来检测、识别和编目所有存在于人类眼表并可能影响视力的微生物。我们将首次对健康眼表的微生物多样性进行全面的表征，这将使我们能够监测健康和患病眼睛的细菌多样性。该项目的成功完成将把眼部感染的诊断和了解提高到一个新的水平。PHS 398/2590(09/04版，2006年4月4日重新发布)页面续格式页面