La Jolla Interdisciplinary Neuroscience Center Cores - Pro Core Salk

拉霍亚跨学科神经科学中心核心 - Pro Core Salk

• 批准号: 8132835
• 负责人: STUART A LIPTON
• 金额: $ 12.19万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8132835
• 关键词: Applications Grants; Award; Blood capillaries; Cancer Center; Communities; Data; Data Set; Databases; Detection; Digestion; Electrospray Ionization; Equipment; Fee-for-Service Plans; Fingerprint; Gel; High Pressure Liquid Chromatography; Human Resources; Immunoprecipitation; Individual; Institutes; Intranet; Ions; Laboratories; Maps; Mass Spectrum Analysis; Measurement; Measures; Medical Research; Neurobiology; Neurosciences; Pathway interactions; Peptides; Phosphorylation; Post-Translational Protein Processing; Proteins; Proteolysis; Proteomics; Proteomics Shared Resource; Protocols documentation; Research Institute; Research Personnel; Solutions; System; Work; authority; base; capillary; glycosylation; instrument; instrumentation; mass spectrometer; meetings; protein aminoacid sequence; protein complex; small molecule; synthetic peptide; tandem mass spectrometry

A primary objective for this award is to integrate the proteomics efforts of the La Jolla Neurobiology community into the existing Proteomics Center at the Burnham Institute for Medical Research. This will involve the purchase of additional instrumentation (an LTQ Ion Trap in Year 1), and the hiring of additional personnel. By amalgamating the neuroscience Proteomics Shared Resource with that of the current Center (which includes the Proteomics Facility for the Burnham's Cancer Center as well as the NTCNP Center on Proteolytic Pathways), we will be able to capitalize on the considerable instrumentation and expertise of the existing Center for the benefit of the Neuroscience community. The current grant application will facilitate equal access to these facilities by Neuroscientists, who do not have access currently because the facility is in the Cancer Center. The integration of the Neuroscience proteomics effort into the existing Proteomics Center will provide a substantial jump-start for the Neuroscience proteomics effort. In addition to the current equipment at the Burnham Institute for Medical Research (BIMR) listed above, which is under the direction of Dr. Jeff Smith, satellite facilities are also available at The Salk Institute (under the direction of Wolfgang Fischer) and at The Scripps Research Institute (TSRI), under the direction of noted proteomics authority John Yates, III. For example, when required for larger proteins or for very precise measurements of mass, the recently-installed Finnigan LTQ/FT instrument at TSRI will be made available to neuroscientists. A technician at TSRI will be designated to specifically interact with and meet the needs of the Neuroscience Community in this regard. Additionally, at the satellite proteomics facility at The Salk Institute, The Bruker Ultraflex Mass Spectrometer has an interface for sequencing peptides on SELDI CHIPs initially characterized by smaller Chiphergen systems located in individual laboratories at the BIMR, Salk, or TSRI. Hence, the three facilities at BIMR (hub facility) and Salk and TSRI (hub facilities) will work together to allow each other's equipment to be used for maximal efficiency. Drs. Smith, Yates, and Fischer will meet monthly to discuss usage, prioritize projects, and insure that neuroscientists have access.

该奖项的主要目的是整合拉霍亚神经生物学的蛋白质组学成果 社区现有的蛋白质组学中心在伯纳姆医学研究所。这将 涉及购买额外的仪器（第1年的LTQ离子阱），并雇用 额外的人员。通过将神经科学蛋白质组学共享资源与 目前的中心（包括伯纳姆癌症中心的蛋白质组学设施以及 NTCNP蛋白水解途径中心），我们将能够利用相当大的 仪器和现有中心的专业知识，为神经科学界的利益。的 目前的拨款申请将促进神经科学家平等地使用这些设施， 目前可以访问，因为该设施位于癌症中心。神经科学的整合 现有的蛋白质组学中心的蛋白质组学工作将提供一个实质性的启动， 神经科学蛋白质组学研究。除了伯纳姆研究所现有的设备外， 上面列出的医学研究（BIMR），由Jeff Smith博士指导， 也可在索尔克研究所（在沃尔夫冈菲舍尔的指导下）和斯克里普斯 研究所（TSRI），在著名的蛋白质组学权威约翰耶茨，三。为 例如，当需要较大的蛋白质或非常精确的质量测量时， TSRI最近安装的Finnigan LTQ/FT仪器将提供给神经科学家。 TSRI的一名技术人员将被指定专门与 神经科学在这方面此外，在索尔克的卫星蛋白质组学设施中， Bruker Ultraflex质谱仪具有用于SELDI上肽测序的接口 CHIP最初的特点是位于各个实验室的较小的Chiphergen系统， BIMR、Salk或TSRI。因此，BIMR（枢纽设施）和Salk和TSRI（枢纽设施）的三个设施 将共同努力，使彼此的设备得到最大的效率。史密斯医生耶茨医生 费舍尔和费舍尔将每月开会讨论使用情况，优先考虑项目，并确保神经科学家 access.