HIGH-THROUGHPUT SOLUTION SCATTERING DATA COLLECTION SYSTEM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们继续开发用于溶液X射线散射的自动化高通量数据采集系统的仪器，结合了石英毛细管池和电动换样器，该换样器包括一个标准96孔微孔板格式的温控样品架和三个电动微型线性平移工作台，其垂直臂固定着用于样品等分选择和输送的注射针。汉密尔顿560液体分配器带有两个微型注射器和专用阀门，用于吸管样品等分并将清洁溶液送到毛细管室。我们最近开发了一个新的数据收集选项卡，名为？SolSAXS？在Blu-Ice中控制硬件并运行自动溶液分散实验。自动化序列消除了人工更换样品，并导致高效地利用射束时间。我们已经成功地结合了一种光纤UV/VIS。用于在一系列X射线测量后立即在原位在线测定蛋白质浓度的分光光度计。该系统正处于调试的最后阶段，同时我们还在继续完善硬件和软件组件。该系统通常每次测量需要25微升样品等分，平均约为25微升。每个样品3.5分钟，包括10次2秒的曝光和对毛细管池的广泛清洁。我们已经开始重新设计一些关键部件，以提高可靠性、等分定位精度和适用性。SolSAXS选项卡继续得到改进和完善。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We continue developing the instrumentation for an automated high throughput data collection system for solution x-ray scattering, combining a quartz capillary cell and a motorized sample changer, which comprises a temperature-controlled sample rack in the standard 96-well microplate format and three motorized miniature linear translation stages whose vertical arm holds a syringe needle for sample aliquot selection and delivery. A Hamilton 560 fluid dispenser with two microsyringes and dedicated valves is integrated for pipeting sample aliquots and sending cleaning solutions to the capllary cell. We have recently developed a new data collection tab named ?SolSAXS? in Blu-Ice to control the hardware and to run automated solution scattering experiments. The automated sequence eliminates manual sample change, and leads to highly efficient use of beam time. We have successfully incorporated a fiber-optic UV/vis. spectrophotometer for on-line determination of protein concentration in situ immediately following a series of x-ray measurements. The system is in the final stage of commissioning while we continue to refine hardware and software components. The system typically requires 25 microliters of sample aliquot per measurement and averages approx. 3.5 minutes per sample, including ten 2s exposures and extensive cleaning of the capillary cell. We have begun redesigning some of the key components for improved reliability, aliquot position accuracy and serviceability. The SolSAXS tab continues to receive improvements and refinements.