HIGH-THROUGHPUT SOLUTION SCATTERING DATA COLLECTION SYSTEM

高通量解决方案散射数据采集系统

• 批准号: 8169999
• 负责人: ANNE MARTEL
• 金额: $ 8.14万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169999
• 关键词: Aliquot; Blood capillaries; Cells; Computer Retrieval of Information on Scientific Projects Database; Computer software; Data Collection; Fiber Optics; Funding; Grant; Ice; In Situ; Institution; Liquid substance; Manuals; Measurement; Names; Needles; Positioning Attribute; Proteins; Quartz; Research; Research Personnel; Resources; Running; Sampling; Series; Solutions; Source; Staging; Syringes; System; Temperature; Time; Translations; United States National Institutes of Health; arm; capillary; improved; instrumentation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We continue developing the instrumentation for an automated high throughput data collection system for solution x-ray scattering, combining a quartz capillary cell and a motorized sample changer, which comprises a temperature-controlled sample rack in the standard 96-well microplate format and three motorized miniature linear translation stages whose vertical arm holds a syringe needle for sample aliquot selection and delivery. A Hamilton 560 fluid dispenser with two microsyringes and dedicated valves is integrated for pipeting sample aliquots and sending cleaning solutions to the capllary cell. We have recently developed a new data collection tab named ?SolSAXS? in Blu-Ice to control the hardware and to run automated solution scattering experiments. The automated sequence eliminates manual sample change, and leads to highly efficient use of beam time. We have successfully incorporated a fiber-optic UV/vis. spectrophotometer for on-line determination of protein concentration in situ immediately following a series of x-ray measurements. The system is in the final stage of commissioning while we continue to refine hardware and software components. The system typically requires 25 microliters of sample aliquot per measurement and averages approx. 3.5 minutes per sample, including ten 2s exposures and extensive cleaning of the capillary cell. We have begun redesigning some of the key components for improved reliability, aliquot position accuracy and serviceability. The SolSAXS tab continues to receive improvements and refinements.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们继续开发用于溶液X射线散射的自动化高通量数据收集系统的仪器，该系统结合了石英毛细管池和电动进样器，该进样器包括标准96孔微孔板格式的温控样品架和三个电动微型线性平移台，其垂直臂固定用于样品等分试样选择和递送的注射器针头。集成了带有两个微量注射器和专用阀的汉密尔顿560液体分配器，用于移液样品等分试样并将清洁溶液发送到毛细管池。我们最近开发了一个新的数据收集选项卡，名为？SolSAXS？在Blu-Ice中控制硬件并运行自动化溶液散射实验。自动化序列消除了手动样品更换，并导致射束时间的高效利用。我们已经成功地将光纤紫外可见维斯。分光光度计，用于在一系列X射线测量后立即在线原位测定蛋白质浓度。该系统正处于调试的最后阶段，同时我们继续完善硬件和软件组件。 该系统通常每次测量需要25微升的样品等分试样，平均值约为100微升。3.5每个样品需要10分钟，包括10次2s暴露和毛细管池的大量清洁。我们已经开始重新设计一些关键部件，以提高可靠性、等分位置精度和可维修性。SolSAXS选项卡将继续得到改进和完善。