CHARACTERIZATION OF ANTIGENIC PEPTIDES PRESENTED BY I-AG7

I-AG7 呈现的​​抗原肽的表征

• 批准号: 8168793
• 负责人: EMIL Raphael UNANUE
• 金额: $ 0.62万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168793
• 关键词: Cataloging; Catalogs; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Histocompatibility Antigens Class II; Inbred NOD Mice; Institution; Mass Spectrum Analysis; Mouse Cell Line; Peptides; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; improved; insulinoma; technique development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to identify peptides presented by the diabetogenic MHC class II molecule I-Ag7 from NOD mice. Studies will use both NOD mice and cell lines derived from insulinomas. The study will extend to cover the effect of loading, posttranslational modification, and challenge by foreign peptides and proteins. Mass spectrometry will be used to sequence and catalog these peptides and the development of techniques to improve sensitivity and analysis will be pursued.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目标是鉴定NOD小鼠致糖尿病MHC II类分子I-Ag 7所提呈的肽。 研究将使用NOD小鼠和来自胰岛素瘤的细胞系。 这项研究将扩展到包括负载，翻译后修饰和外来肽和蛋白质的挑战的影响。 质谱法将被用来对这些肽进行测序和编目，并将继续开发提高灵敏度和分析的技术。