Automated Biodosimetry

自动生物剂量测定

• 批准号: 8172179
• 金额: $ 69.86万
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8172179
• 关键词: Area; Artificial Intelligence; Automation; Biological Assay; Biological Markers; Biological Neural Networks; Cytogenetics; Devices; Dicentric chromosome; Documentation; Dose; Evaluation; Event; Fatigue; Hour; Human; Human Resources; Image Analysis; Individual; Laboratories; Medical; Metaphase; Modeling; Nuclear; Process; Radiation; Risk; Robotics; Sampling; State Government; Stratification; System; Systems Analysis; Testing; Time; Triage; United States; Variant; base; biodosimetry; cohort; dosimetry; handheld mobile device; metropolitan; micronucleus; tool; treatment response

The Unites States Government and Federal agencies have estimated that over 1 million people may seek information on their personal risk (radiation exposure level), if an event (such as a 10 kiloton detonation of an improvised nuclear device) occurs in a large metropolitan area. There will be a need for immediate triage and rapid cytogenetic biodosimetry for estimation of whole-body dose (<2Gy, 2-4Gy, >4Gy) of individuals within 48 hours, to determine and categorize the exposed cohorts for dose based stratification and effective medical management. Individuals exposed at levels exceeding 2 Gy will need immediate treatment and further evaluation with more sophisticated and precise dosimetry tools (such as devices, bioassays, biomarkers etc) to ascertain their absorbed dose. Several of these established available assays are very labor intensive (demands skilled personnel; cytogeneticist) and time consuming (days to weeks), which serves to be a bane during a mass-casualty event. Effective medical management, response and treatment to the exposed cohort (within a short time window) can only be maximized and achieved with the aid of robotic tools and automated system. Automated cytogenetic systems can also reduce significant level of human error caused by fatigue due to the magnitude of samples to be processed. The Automated Cytogenetics Laboratory at AFRRI, focuses on automation of various classical biodosimerty assays (Dicentric Chromosomes, Micronuclei, PCC assays etc) to effectively enhance the throughput of sample analysis, thereby medical management.

联合州政府和联邦机构估计，如果大型大都市地区发生事件（例如10千座爆炸），则超过100万人可能会寻求有关其个人风险（辐射暴露水平）的信息（辐射暴露水平）。在48小时内，将需要立即分类和快速的细胞遗传生物测量法，以估算48小时内的全身剂量（<2gy，2-4gy，> 4GY）的个体，以确定和分类基于剂量的分层和有效医疗管理的暴露同类。以超过2 Gy的水平暴露的个体需要立即治疗，并使用更精致和精确的剂量测定工具（例如设备，生物测定，生物标志物等）来确定其吸收剂量。 这些已建立的可用测定中有几个是非常劳动密集的（需求熟练的人员；细胞遗传学家）和耗时（天到几周），这是在大规模休闲事件中是祸根。只有在机器人工具和自动化系统的帮助下才能最大化和实现对裸露队列的有效医疗管理，反应和治疗（在短时间内）。自动化的细胞遗传学系统还可以减少由于要处理的样品的大小而导致疲劳引起的显着水平的人体错误。 AFRRI的自动化细胞遗传学实验室的重点是自动化各种经典的生物象征分析（二齿染色体，微核，PCC分析等），以有效增强样品分析的吞吐量，从而从中进行医学管理。