Diagnostic Influenza RT-PCR Array

诊断流感 RT-PCR 芯片

• 批准号: 8094348
• 负责人: DARRELL P CHANDLER
• 金额: $ 98.56万
• 依托单位: AKONNI BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8094348
• 关键词: Area; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Communicable Diseases; Computer software; Data Analyses; Data Reporting; Detection; Development; Diagnosis; Diagnostic; Disease; Disease Outbreaks; Elements; Far East; Goals; Health; Hospitalization; Hospitals; Hour; Image; Influenza; Influenza A Virus, H5N1 Subtype; Institution; Microarray Analysis; Performance; Peripheral; Pharmaceutical Preparations; Phase; Preparation; RNA Viruses; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Study Section; Swab; System; Technology; Testing; United States Food and Drug Administration; Universities; Viral; blind; cost; influenzavirus; pandemic disease; particle; pre-clinical; research study

DESCRIPTION (provided by applicant): Influenza viruses are highly contagious negative-sense RNA viruses cause approximately 114,000 hospitalizations and 20,000 deaths annually. Several drugs are approved by the US Food and Drug Administration (FDA) for treating influenza infections, but successful treatment depends on knowing the specific antigenic subtype and administering these drugs within the first 48 h of illness. Recent outbreaks of avian influenza H5N1 in East Asia have fueled concern over another pandemic and emphasize the need for comprehensive strain sub-typing in surveillance and diagnostic applications. The objective of this Phase 2 Project is therefore to develop and field test a sample-to-answer RT-PCR array kit for influenza detection and antigenic sub-typing in diagnostic and surveillance settings, with an emphasis on simple, low-cost microarray technology that can be deployed and used in peripheral hospital labs. The performance goal is a sample-to answer result in 2 hours or less at an input target concentration of 102 viral particles per mL nasopharyngeal swab. Early adopters at Wadsworth Center, Columbia University, Little Company of Mary Hospital and the CDC will assist in the pre-clinical verification of the technology on amended and blind clinical samples. PUBLIC HEALTH RELEVANCE: The influenza RT-PCR array will be the first-of-its-kind combination of PCR and microarray technology in a simple, inexpensive test kit for disease surveillance and diagnosis. The underlying platform developed herein will likewise find broad application in many areas of infectious disease diagnostics.

描述（由申请人提供）：流感病毒是高度传染性的负义RNA病毒，每年导致大约114,000人住院，20,000人死亡。美国食品和药物管理局（FDA）批准了几种治疗流感感染的药物，但成功的治疗取决于了解特定的抗原亚型，并在发病的头48小时内使用这些药物。最近在东亚暴发的H5N1禽流感加剧了人们对另一次大流行的担忧，并强调在监测和诊断应用中需要进行全面的毒株分型。因此，第二阶段项目的目标是开发和现场测试一种样本到答案的RT-PCR阵列试剂盒，用于流感检测和诊断和监测环境中的抗原亚型分型，重点是可在外围医院实验室部署和使用的简单、低成本微阵列技术。性能目标是在输入目标浓度为每毫升鼻咽拭子102个病毒颗粒的情况下，在2小时或更短的时间内得到样本应答结果。沃兹沃斯中心、哥伦比亚大学、玛丽医院的小公司和疾病预防控制中心的早期采用者将协助该技术在修改后的盲临床样本上进行临床前验证。公共卫生相关性：流感RT-PCR阵列将是首个将PCR和微阵列技术结合在一种简单、廉价的疾病监测和诊断检测试剂盒中的同类产品。本文开发的基础平台同样将在传染病诊断的许多领域得到广泛应用。

项目成果

Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection.

开发和临床测试一种简单的低密度凝胶元件阵列，用于流感鉴定、亚型分型和 H275Y 检测。

• DOI: 10.1016/j.jviromet.2014.07.019
• 发表时间: 2014
• 期刊: Journal of virological methods
• 影响因子: 3.1
• 作者: Chandler,DarrellP;Griesemer,SaraB;Knickerbocker,Christopher;Golova,JuliaB;Lambarqui,Amine;Perov,AlexanderN;Zimmerman,Cynthia;Wiles,Cory;Rudy,GeorgeB;StGeorge,Kirsten
• 通讯作者: StGeorge,Kirsten

Integrated Amplification Microarrays for Infectious Disease Diagnostics.

用于传染病诊断的集成扩增微阵列。

• DOI: 10.3390/microarrays1030107
• 发表时间: 2012
• 期刊: Microarrays (Basel, Switzerland)
• 作者: Chandler,DarrellP;Bryant,Lexi;Griesemer,SaraB;Gu,Rui;Knickerbocker,Christopher;Kukhtin,Alexander;Parker,Jennifer;Zimmerman,Cynthia;George,KirstenSt;Cooney,ChristopherG
• 通讯作者: Cooney,ChristopherG