Autoantibody and Autoantigen Biomarker Discovery Kits for the Research Community

说明(申请人提供)：免疫球蛋白占人类血浆蛋白的30%-50%，在许多疾病中可以表现出几倍的水平增加。“天然”自身抗体可能是免疫稳态机制的一部分，可能占健康人血浆的50%。因此，疾病相关抗体的鉴别必须能够有效地减去一个大而特殊的“背景”成分。 虽然自身抗体和疾病是重要的和研究得很好的，但在这一领域仍有许多工作要做，涉及许多疾病和潜在的应用。蛋白质组学研究人员越来越多地参与到生产可靠试剂和共享试剂的工作中来。 该项目将为自身抗体和自身抗原生物标记物的发现提供一种新的研究工具，作为一种结合差异捕获蛋白质组学技术的试剂盒提供给科学研究界，以减少非疾病特异性结合。 这一多功能试剂盒将把经过验证的模块化和现有试剂盒组件与创新方法相结合，并将提供一种实验上简单但功能强大的工具，用于探索广泛的抗体和抗原生物标记物发现项目。 因此，将向研究界提供自身抗体和自身抗原生物标记物发现试剂盒，希望双向交流的想法和试剂可以为涉及自身抗体的各种疾病的这种努力做出贡献。 特定目的1：研制试剂盒组件1，分离与疾病相关抗体结合的噬菌体多肽 特定目的2：开发试剂盒组件2，以分离与疾病相关抗体结合的多肽 特定目的3：开发试剂盒组件3，以分离与疾病相关抗原结合的抗体珠 公共卫生相关性：虽然自身抗体和疾病很重要且研究得很好，但在这一领域仍有许多工作要做，这涉及许多疾病和潜在的应用。 该项目将为自身抗体和自身抗原生物标记物的发现提供一种新的研究工具，作为一种结合差异捕获蛋白质组学技术的试剂盒提供给科学研究界，以减少非疾病特异性结合。

DESCRIPTION (provided by applicant): Immunoglobulins comprise 30-50% of human plasma proteins, and can show several- fold increased levels in many diseases. "Natural" autoantibodies, presumably part of an immune homeostasis mechanism, may comprise >50% of a healthy individual's plasma. The differential identification of disease-associated antibodies must therefore be able to effectively subtract a large and idiosyncratic "background" component. Although autoantibodies and disease are important and well-studied, much work remains to be done in this area, which involves many diseases and potential applications. Proteomics researchers are increasingly involved in generating reliable reagents and in sharing them. This project will deliver a novel research tool for autoantibody and autoantigen biomarker discovery, to be provided for the scientific research community as a kit that incorporates Differential Capture Proteomics technology to subtract non disease-specific binding. This versatile kit will integrate proven modular and existing kit components with innovative methodologies, and will provide an experimentally straightforward but powerful tool to explore a wide range of antibody and antigen biomarker discovery projects. The Autoantibody and Autoantigen Biomarker Discovery Kit will therefore be offered to the Research Community in the hope that a two-way flow of ideas and reagents may contribute to such efforts in a variety of diseases involving autoantibodies. Specific Aim 1: Develop Kit Component 1, to isolate phage peptides binding disease-associated antibodies Specific Aim 2: Develop Kit Component 2, to isolate peptide beads binding disease-associated antibodies Specific Aim 3: Develop Kit Component 3, to isolate antibody beads binding disease-associated antigens PUBLIC HEALTH RELEVANCE: Although autoantibodies and disease are important and well-studied, much work remains to be done in this area, which involves many diseases and potential applications. This project will deliver a novel research tool for autoantibody and autoantigen biomarker discovery, to be provided for the scientific research community as a kit that incorporates Differential Capture Proteomics technology to subtract non disease-specific binding.