Autoantibody and Autoantigen Biomarker Discovery Kits for the Research Community

描述（由申请人提供）：免疫球蛋白占人血浆蛋白的30-50%，在许多疾病中其水平可增加几倍.“天然”自身抗体，可能是免疫稳态机制的一部分，可包含>50%的健康个体血浆。因此，疾病相关抗体的差异鉴定必须能够有效地减去大的特异质“背景”组分。 虽然自身抗体和疾病是重要的和充分的研究，在这一领域仍然有很多工作要做，这涉及到许多疾病和潜在的应用。蛋白质组学研究人员越来越多地参与生产可靠的试剂并分享它们。 该项目将为自身抗体和自身抗原生物标志物的发现提供一种新的研究工具，作为一种试剂盒提供给科学研究界，该试剂盒结合了差异捕获蛋白质组学技术，以减少非疾病特异性结合。 这种多功能试剂盒将整合成熟的模块化和现有的试剂盒组件与创新的方法，并将提供一个实验简单，但强大的工具，探索广泛的抗体和抗原生物标志物发现项目。 因此，将向研究界提供自身抗体和自身抗原生物标志物发现试剂盒，希望双向流动的想法和试剂可能有助于在涉及自身抗体的各种疾病中进行此类努力。 具体目标1：开发试剂盒组分1，以分离结合疾病相关抗体的噬菌体肽 具体目标2：开发试剂盒组分2，以分离结合疾病相关抗体的肽珠 具体目标3：开发试剂盒组分3，以分离结合疾病相关抗原的抗体珠 公共卫生相关性：虽然自身抗体和疾病是重要的和充分的研究，在这一领域仍然有很多工作要做，这涉及到许多疾病和潜在的应用。 该项目将为自身抗体和自身抗原生物标志物的发现提供一种新的研究工具，作为一种试剂盒提供给科学研究界，该试剂盒结合了差异捕获蛋白质组学技术，以减少非疾病特异性结合。

DESCRIPTION (provided by applicant): Immunoglobulins comprise 30-50% of human plasma proteins, and can show several- fold increased levels in many diseases. "Natural" autoantibodies, presumably part of an immune homeostasis mechanism, may comprise >50% of a healthy individual's plasma. The differential identification of disease-associated antibodies must therefore be able to effectively subtract a large and idiosyncratic "background" component. Although autoantibodies and disease are important and well-studied, much work remains to be done in this area, which involves many diseases and potential applications. Proteomics researchers are increasingly involved in generating reliable reagents and in sharing them. This project will deliver a novel research tool for autoantibody and autoantigen biomarker discovery, to be provided for the scientific research community as a kit that incorporates Differential Capture Proteomics technology to subtract non disease-specific binding. This versatile kit will integrate proven modular and existing kit components with innovative methodologies, and will provide an experimentally straightforward but powerful tool to explore a wide range of antibody and antigen biomarker discovery projects. The Autoantibody and Autoantigen Biomarker Discovery Kit will therefore be offered to the Research Community in the hope that a two-way flow of ideas and reagents may contribute to such efforts in a variety of diseases involving autoantibodies. Specific Aim 1: Develop Kit Component 1, to isolate phage peptides binding disease-associated antibodies Specific Aim 2: Develop Kit Component 2, to isolate peptide beads binding disease-associated antibodies Specific Aim 3: Develop Kit Component 3, to isolate antibody beads binding disease-associated antigens PUBLIC HEALTH RELEVANCE: Although autoantibodies and disease are important and well-studied, much work remains to be done in this area, which involves many diseases and potential applications. This project will deliver a novel research tool for autoantibody and autoantigen biomarker discovery, to be provided for the scientific research community as a kit that incorporates Differential Capture Proteomics technology to subtract non disease-specific binding.