Multi-Blot Western Device

多重印迹蛋白质印迹设备

• 批准号: 8642501
• 负责人: Stanley Yung-Ping Chien
• 金额: $ 30.78万
• 依托单位: YC BIOELECTRIC, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8642501
• 关键词: Anodes; Antibodies; Automation; Biological Sciences; Biomedical Engineering; Capillary Electrophoresis; Clinical; Computer software; Computers; Corrosion; Detection; Development; Devices; Diagnostic; Doctor of Philosophy; Drug Industry; Electrodes; Feasibility Studies; Gel; Generations; Goals; Health; Image; Indiana; Investigation; Iridium; Licensing; Life; Location; Marketing; Membrane; Molecular Weight; Output; Personal Computers; Phase; Phosphotransferases; Physiologic pulse; Preparation; Prevention; Procedures; Process; Productivity; Protein Isoforms; Proteins; Quality Control; Reaction; Research; Resources; Running; Safety; Sampling; Scientist; Shapes; Signal Pathway; Signal Transduction; Signaling Protein; Small Business Technology Transfer Research; Software Design; Software Tools; Solutions; Speed; Surface Properties; System; Techniques; Technology; Testing; Time; Titania; Titanium; Universities; Variant; Western Blotting; Width; base; commercialization; cost; design; electric field; improved; innovation; novel; phase 2 study; prevent; programs; prototype; research study; simulation; tool; user friendly software; user-friendly; voltage

DESCRIPTION (provided by applicant): The long-term objective of this STTR project is to commercialize the protein multi-blot Western device, which contributes to improving the productivity and accuracy of conventional Western blot analysis. Although Western blotting has been used for more than three decades, this labor-and-cost demanding technique remains a key routine for a majority of life scientists. To improve Western blot analysis, new products for quick antibody reactions, better quantitation through imaging and software, and capillary electrophoresis for fully automation have been released. However, few products are directly targeted to improve a traditional blotting process which is still widely used. In this project, YC Bioelectric, LLC (YCB) proposes to develop a novel multi-blot Western device, which enables multiple copies of protein species of varying sizes from a single electrophoretic gel. In this device, a protein transfer unit utilizes multiple anodes to generate a non-uniform voltage profile in order to enable uniform protein transfer speed regardless of protein size. A preliminary study was conducted at Indiana University, and results show that the expression levels different proteins are detectable from each of 5-7 membranes made from a single gel. YCB was founded in June 2012 to manufacture a marketable multi-blot Western device product. YCB currently holds an option to take an exclusive license from the Indiana University Research and Technology Commercialization. YCB and Indiana University propose to extend the current proof-of-concept investigation to a full feasibility study. The hypothesis is that multi-blotting utilizing a multi-anode plate not only enhances accuracy of Western blot analysis but also significantly reduces cost and labor. The hypothesis will be tested with the following specific aims: Aim 1: Fabrication of the refined blotting hardware; Aim 2: Implementation of the enhanced control system; Aim 3: Development of a predictive software tool The revised proposal includes the development of a novel one button operated imaging based blotting control subsystem and an inclusion of a predictive software tool. Furthermore, solutions to four technical weaknesses in the last review regarding a voltage gradient, anode materials, quality control, and gel handling, are presented. According to its market analysis, YCB expects that its target customers in life science labs will appreciate the proposed multi-blot Western device because of its capability of saving costs, time, and samples with a user-friendly predictive software tool.

描述（由申请人提供）：该STTR项目的长期目标是商业化蛋白质多印象西部装置，这有助于提高常规蛋白质印迹分析的生产率和准确性。尽管Western印迹已被使用了三十多年，但这种劳动和成本苛刻的技术仍然是大多数生活科学家的关键常规。为了改善Western印迹分析，已释放用于快速抗体反应，通过成像和软件进行更好定量的新产品以及用于完全自动化的毛细管电泳。但是，很少有产品直接用于改善仍被广泛使用的传统印迹过程。在这个项目中，YC Bioelectric，LLC（YCB）提议开发一种新型的多墨西哥西部装置，该设备可以从单个电泳凝胶中获得多种不同大小的蛋白质种类。在该设备中，蛋白质转移单元利用多个阳极生成不均匀的电压曲线，以使均匀的蛋白质转移速度无论蛋白质大小如何。在印第安纳大学进行了一项初步研究，结果表明，从单个凝胶制成的5-7个膜中，可以检测到不同蛋白质的表达水平。 YCB成立于2012年6月，旨在生产可销售的多印象西方设备产品。 YCB目前拥有从印第安纳大学研究和技术商业化获得独家许可证的选项。 YCB和印第安纳大学建议将当前的概念验证调查扩展到一项完整的可行性研究。假设是，利用多动力板的多种印迹不仅提高了Western印迹分析的准确性，而且可以显着降低成本和劳动。该假设将通过以下特定目的进行检验：目标1：精制印迹硬件的制造；目标2：增强控制系统的实施； AIM 3：开发预测软件工具修订的建议包括开发基于成像的新型播放成像子系统和包含预测软件工具的新型按钮。此外，提出了关于电压梯度，阳极材料，质量控制和凝胶处理的最后评论中四个技术弱点的解决方案。根据其市场分析，YCB预计其生命科学实验室中的目标客户将欣赏拟议的多框西方设备，因为它可以使用用户友好的预测软件工具节省成本，时间和样品。