Histomics

项目摘要 该视觉研究核心（VRC）的总体目标是提供： ·获得个别R01奖励范围以外的资源 ·获得单一实验室范围之外的技术专长 ·员工培训，以消除有效转化研究和合作的障碍 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发展障碍，青光眼和其他疾病，以及和一系列尖端的基本 科学倡议。我们已经实现了四个资源模块，继续自然演变， 这个研究小组如何协同工作，为15名研究人员提供21项NEI R01奖。 组织学模块来自于莫兰眼科中心在小鼠遗传学方面的优势， 对组织分析的高要求。几乎每一个JMEC实验室都需要高质量的“组织学” （冷冻切片、对位、基因分型、免疫细胞化学、PCR、ISH）。 具体而言，Histomics模块提供： ·通过两个专业维护的Leica CM3050 S系统提供高质量的冷冻切片资源 ·通过新型Leica Paratome进行石蜡包埋和切片服务 · PCR和基因分型服务 ·小鼠冻存线服务

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 15 investigators holding 21 NEI R01 awards. The Histomics Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue profiling. Almost every JMEC laboratory has need of high quality “histomics” (cryosectioning, paratoming, genotyping, immunocytochemistry, PCR, ISH). Specifically the Histomics module provides: · High-quality cryotomy resources through two professionally maintained Leica CM3050S systems · Paraffin embedding and section services via a new Leica Paratome · PCR and genotyping services · Mouse line cryopreservation services