Core B -Comprehensive Mouse Physiology and Phenotyping

工作总结：CORE B-综合小鼠生理学和表型 全面的小鼠生理学和小鼠表型，包括压力超负荷的外科模型 导致心肌肥大和心力衰竭。PI的实验室在广泛的领域拥有相当多的专业知识 为小鼠的表型鉴定提供方法学指导，并将为PPG成员提供核心服务。《少年派》 还参与了许多心力衰竭遗传和外科模型的开发。为 例：压力超负荷心肌肥厚的显微外科手术和血流动力学评估 经腹主动脉横断收缩诱导，是由PI发展而来的，现在被认为是一种常规 程序在世界各地的许多实验室。经胸二维引导M型超声心动图，血流动力学 而通过压力-体积环和应力应变分析小鼠的收缩功能是标准程序， 其中许多都是在PI的实验室里开发的。游泳和跑轮子相结合的运动训练 老鼠是目前正在使用的方法。

SUMMARY OF WORK: Core B – Comprehensive Mouse Physiology and Phenotyping Comprehensive mouse physiology and mouse phenotyping including surgical models of pressure overload to induce cardiac hypertrophy and heart failure. The PI's laboratory has considerable expertise in a wide-range of methodologies for the phenotyping of mice and will provide Core service to members of the PPG. The PI has also been involved in the development of numerous genetic and surgical models of heart failure. For example, microsurgical procedures and hemodynamic evaluation of pressure overload cardiac hypertrophy induced through transverse aortic constriction was developed by the PI, and is now considered a routine procedure in many labs around the world. Transthoracic 2D guided M-mode echocardiography, hemodynamic and contractile function by pressure-volume loops and stress strain analyses in mice are standard procedures, many which were developed in the PI's laboratory. Exercise training by both swimming and wheel running for mice are methods currently being used.