The importance of host cell invasion and type III secretion to Campylobacter rectus virulence

宿主细胞侵袭和 III 型分泌对直肠弯曲杆菌毒力的重要性

• 批准号: 8968447
• 负责人: DEBORAH S THREADGILL
• 金额: $ 10.91万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8968447
• 关键词: Adult; Affect; Age; Age-Months; Animals; Antigens; Bacteremia; Bacteria; Blood specimen; Campylobacter; Campylobacter jejuni; Campylobacter rectus; Cells; Complement; DNA; Data; Disease; Enteral; Fetus; Future; Gastrointestinal tract structure; Genes; Genetic; Gingivitis; Goals; Health; Homologous Gene; Human; Individual; Induction of Apoptosis; Infection; Inflammatory Bowel Diseases; Invaded; Livestock; Low Birth Weight Infant; Molecular; Needles; Oral; Oral cavity; Pathogenesis; Patients; Periodontal Diseases; Periodontitis; Phenotype; Positioning Attribute; Pregnancy Outcome; Process; Protein Secretion; Proteins; Regulation; Relative (related person); Research; Research Personnel; Research Project Grants; Risk Factors; Shapes; Source; Surface; System; Systemic disease; Term Birth; Testing; Tissues; Type II Secretion System Pathway; Type III Secretion System Pathway; Virulence; Woman; complement system; functional genomics; human disease; mutant; oral bacteria; pathogen; pathogenic bacteria; peripheral blood; public health relevance; reproductive; retinal rods

 DESCRIPTION (provided by applicant): Campylobacter rectus is a rod-shaped Gram-negative anaerobic oral bacterium that has been identified in 90% of adults with initial or established periodontitis, compared with 10% of healthy individuals and 20% of individuals with gingivitis. In addition to a clear association with periodontal disease, recent studies have highlighted the involvement of C. rectus infection in Barrett's esophoagus, oral and extraoral abcesses, inflammatory bowel disease and adverse pregnancy outcomes in humans including pre-term birth and low birth weight. Despite the frequent isolation of C. rectus from humans little is known about the molecular mechanisms of pathogenesis associated with this emerging pathogen. In order to begin an analysis of C. rectus pathogenesis, we will investigate whether invasion and invasion-related genes involved in Campylobacter jejuni pathogenesis are also responsible for C. rectus pathogenesis. In particular, we will 1) Determine if the C. rectus gene annotated as ciaB [Campylobacter invasion antigen B] is a functional homolog to C. jejuni ciaB; 2) Determine the secretion status of C. rectus ciaB and thereby establish whether a functional flagellar type II secretion system exists in C. rectus. Results gained from this small project will support future functional genomic studies of C. rectus on a larger scale to understand how C. rectus contributes to human disease.

 描述（由申请方提供）：直弯曲杆菌是一种杆状革兰氏阴性厌氧口腔细菌，在90%的患有初始或确诊牙周炎的成年人中发现，而在10%的健康个体和20%的牙龈炎个体中发现。除了与牙周病有明确的联系外，最近的研究强调了C。Barrett食管腹直肌感染、口腔和口外脓肿、炎症性肠病和人类不良妊娠结局，包括早产和低出生体重。尽管C.人类的直肌 关于与这种新兴病原体相关的发病机制的分子机制。为了开始对C.直肌发病机制，我们将调查是否入侵和入侵相关基因参与空肠弯曲菌发病机制也负责C。腹直肌发病机制特别是，我们将1）确定是否C。注释为cia B [弯曲杆菌侵袭抗原B]的直肌基因是C的功能同源物。jejuni ciaB; 2）测定C.直肌ciaB，从而确定是否存在功能性鞭毛II型分泌系统在C。直肌本研究的结果将为今后的功能基因组研究提供支持。更大规模的直肌，以了解C。直肌会导致人类疾病