The Midwest Center for Structural Genomics - Community Resource

 描述(由申请人提供)：中西部结构基因组学中心(MCSG)的主要目标是运营高通量蛋白质表征管道，作为生物社区的资源。该平台建立良好，高度集成，成本效益高。包括生物信息学、基因克隆、蛋白质表达、蛋白质纯化和表征、结晶以及使用第三代同步加速器的X射线结晶学的数据收集和结构测定。作为其任务的一部分，MCSG将把其资源用于个人调查人员提名的目标，并将作为美国学术和研究机构的用户设施。MCSG现场有功能齐全的仪器和专业知识来完成这项任务。MCSG将积极与其他科学家互动，进行技术培训，并将使科学界随时获得在该计划过程中产生的所有成果。MCSG位于阿贡国家实验室(ANL)的高级蛋白质表征设施(APCF)，该设施直接连接到位于19区的先进光子源(APS)。APCF是一个最先进的科学合作设施，提供致力于分子、结构和系统生物学的新的专门实验室空间。

 DESCRIPTION (provided by applicant): The primary objective of the Midwest Center for Structural Genomics (MCSG) is to operate the high-throughput protein characterization pipeline as a resource for Biology Community. The platform is well established, highly integrated and cost effective. Is comprised of the following components: bioinformatics, gene cloning, protein expression, protein purification and characterization, crystallization as well as data collection and structure determination using X-ray crystallography at 3rd generation synchrotron. As a part of its mandate, the MCSG will apply its resources for targets nominated by the individual investigators and will serve as a user facility for the U.S. academic and research institutions. The MCSG site has fully functional instrumentation and expertise in place for the task. The MCSG will actively interact with other scientists, conduct technology training and will make all results produced in the course of this program readily available to the scientific community. The MCSG is located in the Advanced Protein Characterization Facility (APCF) at Argonne National Laboratory (ANL) that is directly attached to the Advanced Photon Source (APS) at sector 19. The APCF is a state-of-the-art scientific collaborative facility that provides new, specialized laboratory space devoted to molecular, structural and systems biology.