ShEEP Request for Nanostring nCounter Spring Profiler

ShEEP 请求 Nanostring nCounter Spring Profiler

• 批准号: 9213421
• 负责人: Robert Keith
• 金额: --
• 依托单位: VA EASTERN COLORADO HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9213421
• 关键词: Agonist; Alzheimer' s Disease; Award; Bioinformatics; Blood; Brain; CREB1 gene; Cells; Complementary DNA; Computer software; DNA Sequence; Data; Data Analyses; Detection; Disease; Doctor of Philosophy; FGFR1 gene; Formalin; Gene Chips; Genetic Transcription; Genomics; Hand; Head and Neck Cancer; K-Series Research Career Programs; Kynurenine; Length; Libraries; Malignant Neoplasms; Malignant neoplasm of lung; Marketing; Methods; Molecular Profiling; Neoplasm Metastasis; Oncogenes; Organism; PPAR gamma; Paraffin Embedding; Pathway interactions; Preparation; Principal Investigator; Process; Productivity; RNA; RNA Sequences; Reaction; Request for Applications; Research; Research Institute; Research Personnel; Research Project Grants; Role; Sampling; Specialist; System; Technology; Time; Toxoplasma; Training; Tube; Tuberculosis; Variant; Veterans; abstracting; cancer chemoprevention; cost; design; faculty research; genome-wide; individual patient; instrument; interest; nano-string; personalized medicine; response biomarker; suicidal behavior; tissue fixing; tool; tumor progression; user-friendly; wasting; web site

Abstract In this application we request the purchase of a Nanostring nCounter Sprint Profiler instrument. The nCounter Sprint Profiler is a user friendly, small-footprint, medium throughput instrument designed to detect and quantify up to 800 different RNA or DNA sequences in a single sample. This technology allows expression analysis in a single cell or an entire organism and is designed to bridge the gap between single qPCR reactions and global technologies like expression arrays or genome wide sequencing. This technology represents a major advance in genomic research, by allowing detection and quantification of hundreds of target sequences at much lower cost and time than traditional methods. The following research projects will be supported by the upgrades system: 1) Robert Keith, MD, Principal Investigator: BLR&D Merit Review Award “PPAR gamma Agonists for Lung Cancer Chemoprevention” 2) Howard Li, MD, Principal Investigator: BLR&D Career Development Award “The Role of PPARgamma in Lung Cancer Progression and Metastasis” 3) Nicholas Walter, MD, Principal Investigator: CSR&D Career Development Award “Blood Expression Profiles as a Biomarker for Responses to Tuberculosis” 4) Subbiah Pugazhenthi, PhD, Principal Investigator: BLR&D Merit Review Award “Mechanism of CREB dysregulation in Alzheimer brain” 5) Lynn Heasley, PhD, Principal Investigator: BLR&D Merit Review Award “An FGFR1 oncogene driver pathway in head and neck cancer” 6) Lisa Brenner, PhD, Co-Principal Investigator: CSR&D Merit Review Award “Toxoplasma gondi, the kynurenine pathway, and suicidal behavior in veterans” 7) Edward Chan, MD, Principal Investigator, Merit Review and Denver Research Institute

摘要 在本申请中，我们请求购买一台NanoStringnCounter Sprint Profiler仪器。这个 NCounter Sprint Profiler是一款用户友好、占用空间小、中等吞吐量的仪器，旨在检测 并对单个样本中多达800个不同的RNA或DNA序列进行量化。这项技术允许表达 在单个细胞或整个生物体中进行分析，旨在弥合单个qPCR之间的差距 反应和全球技术，如表达阵列或全基因组测序。这项技术 代表着基因组研究的一个重大进步，通过允许检测和量化数百个 与传统方法相比，靶序列的成本和时间要低得多。以下研究项目将是 受升级系统支持： 1)Robert Keith，医学博士，首席研究员：Blr&D功绩评审奖：肺的PPAR伽马激动剂 癌症的化学预防“ 2)李华德，医学博士，首席研究员：BR&D职业发展奖《PPARGamma的角色》 在肺癌的进展和转移中 3)Nicholas Walter，医学博士，首席研究员：CSR&D职业发展奖《血液表达》 作为结核病反应生物标记物的概况“ 4)Subbiah Pugazhenthi博士，首席研究员：BR&D功绩评审奖《CREB的机制》 阿尔茨海默病的大脑调节失调“ 5)Lynn Heasley，博士，首席研究员：Blr&D功绩评审奖，FGFR1癌基因驱动因素 头颈部癌的途径 6)丽莎·布伦纳(Lisa Brenner)，博士，CSR&D功绩评审奖联合首席研究员 犬尿氨酸途径与退伍军人的自杀行为 7)Edward Chan，医学博士，功绩评论和丹佛研究所首席研究员