Bioinformatics Core

项目总结(见说明)： 作为免疫遗传学系统生物学方法的一部分，生物信息学核心提供数据管理、通信系统、统计分析和对高通量数据的功能解释的支持。将利用现有平台开发和维护复杂的实验室信息管理系统(LIMS)，以促进信息跟踪、通过计算管道处理原始数据以及所有U19调查人员之间的交流。此外，核心将支持根据表达数量性状基因座(EQTL)和QTL分析得出的关键免疫基因和途径的优先顺序。核心的具体目标如下：目标1：开发和维护具有系统免疫遗传网络门户的专用数据管理系统。核心将定义和实施一个项目范围的数据通信计划，以确保捕获所有赠款产生的样本信息、数据和资源。还将通过网络门户网站提供对赠款生成的数据、资源和鼠标系列信息的公开访问。目标2；协助开发数据处理管道，以确保数据高质量，符合建模技术规范，并可供所有U19调查人员使用。与系统免疫遗传核心合作，将利用现有的基础设施为所有高通量简档数据开发和实施数据处理管道，包括微阵列、下一代测序和蛋白质组学的质量控制协议。 目标3：为研究项目和核心产生的高通量数据和计算预测提供分析支持。将为数据和结果的解释提供统计、计算和功能分析支持。此外，Core将提供对下一代测序数据的分析，包括对非编码RNA的深入分析，以及对目标蛋白质组学研究的处理和解释，从而允许从系统层面查看关键免疫基因和选定的一组小鼠品系的路径。

PROJECT SUMMARY (See instructions): The Bioinformatics Core provides data management, communications systems, statistical analysis, and support for functional interpretation of high-throughput data as part of the systems biology approach to immunogenetics. Existing platforms will be leveraged to develop and maintain a sophisticated laboratory information management system (LIMS) to facilitate the tracking of information, the processing of raw data through computational pipelines, and communication between all U19 investigators. In addition, the Core will support the prioritization of key immune genes and pathways derived from expression quantitative trait loci (eQTL) and QTL analyses. The Specific Aims of the Core are as follows: Aim 1: Develop and maintain a dedicated data management system with a Systems Immunogenetics Web Portal. The Core will define and implement a program-wide data communication plan that ensures the capture of all grant-generated sample information, data, and resources. Public access to grant-generated data, resources, and mouse line infomnation will also be provided through the Web portal. Aim 2; Assist in the development of data processing pipelines that ensure data is high-quality, conforms to technical specifications for modeling, and is accessible to all U19 investigators. In collaboration with the Systems Immunogenetics Core, an existing infrastructure will be leveraged to develop and implement data processing pipelines for all high-throughput profiling data, including quality control protocols for microarray, next-generation sequencing, and proteomics. Aim 3: Provide analytical support for high-throughput data and the computational predictions generated by the Research Projects and Cores. Statistical, computational, and functional analysis support will be provided for the interpretation of data and results. In addition, the Core will provide analysis of next-generation sequencing data, including in-depth analysis of noncoding RNAs, and processing and interpretation of targeted proteomics studies allowing for a systems level view of key immune genes and pathways across a select set of mouse lines.