Project 3

项目3。艾滋病毒成功的一个标志是植根于极端的序列可变性，最近的深度测序工作正在显著增加可用数据的数量^^？28)由于其直接与宿主免疫系统相互作用，以往的序列分析大多集中在gpi20序列上。虽然HFV序列存在分化，但它们是在进化压力下分化的，因此，序列是识别单个氨基酸在功能特性中所起作用的强大信息来源。HIV序列变异性不仅影响包膜，而且影响所有HIV蛋白，我们建议将重点放在CA上。CA的主要进化限制与其结构完整性、组装和拆卸过程中的动态特性以及与宿主蛋白的相互作用位点有关。我们计划利用HIV和SIV序列数据进行CA蛋白序列变异性分析，并探讨其对衣壳的影响

Project 3. A hallmark of HIVs success is rooted in extreme sequence variability, and recent deep sequencing efforts are increasing the amount of available data dramatically^^?. 28) Most previous sequence analysis focused on gpi20 sequences due to its direct interaction with the host immune system. While HFV sequences diverge, they do so under evolutionary pressures, and sequences are, therefore, a formidable source of information to identify the role that individual amino acids play in functional properties. HIV sequence variability impacts not only envelope but all HIV proteins, and we propose to focus on CA. The major evolutionary constraints on CA are associated with its structural integrity, its dynamic properties during assembly and disassembly, and its interaction sites with host proteins. We plan to exploit HIV and SIV sequence data to carry out an analysis of CA protein sequence variability and explore its impact on capsid structure, dynamics, and function.