Tissue/Pathology & Immunology Core

该共享资源的目的是收集和分发与宫颈癌和HPV疾病相关的临床注释的人类生物标本，并为约翰霍普金斯/亚拉巴马大学/科罗拉多大学/西奈山宫颈癌孢子的研究人员提供病理学和免疫学专业知识和基于组织的分析支持。组织核心自1998年以来一直存在，并在宫颈癌孢子的支持下扩大。迄今为止，我们已经从810例良性妇科组织、2,220例肿瘤切除术和178例宫颈HPV病变切除术中收集了冷冻组织样本。还获得了入选我们的CIN 2/3方案的245名受试者的纵向脱落细胞和外周血标本，以及良性组织的一次性受试者匹配外周血样本（206）和632份肿瘤样本。福尔马林固定块可用于几乎所有这些标本。此外，这一共享资源还储存了参加HPV相关头颈部鳞状细胞癌治疗性疫苗接种方案的患者的标本。所有的生物标本都是根据国家癌症研究所的生物储存库最佳实践指南收获和储存的。核心已经扩展到包括专家病理学和免疫学咨询研究人员，包括指导定量数字图像分析指导组织的分子研究。标本将在具有妇科病理学专业知识的病理学家的监督下收集，并与这些领域的临床同事密切合作。我们的临床方案中入组的受试者的临床信息被输入到一个有密码保护的基于网络的跟踪系统中。这一基于网络的内部系统遵循国家研究理事会的建议，包括用户身份验证、加密、审计跟踪和灾难恢复。已建立审查机制，以优先向约翰霍普金斯宫颈癌孢子内外的研究者分配所需资源。生物样本已与全国各地学术机构的合作者分享。

The purpose of this shared resource is to collect and distribute clinically annotated human biospecimens related to cervical cancer and HPV disease, and to provide pathologic and immunologic expertise and support for tissue-based analyses for investigators in the Johns Hopkins/University of Alabama/University of Colorado/Mount Sinai Cervical Cancer SPORE. The tissue core has been in existence since 1998, and has expanded with the support of the Cervical Cancer SPORE. To date, we have banked frozen tissue samples from 810 benign gynecologic tissues, 2,220 tumor resections, and 178 resections of preinvasive cervical HPV lesions. Longitudinally obtained exfoliated cell and peripheral blood specimens from 245 subjects enrolled in our CIN2/3 protocols have also been obtained, as well as one-time subject-matched peripheral blood samples for benign tissues (206), and from 632 tumor samples. Formalin fixed blocks are available for virtually all of these specimens. In addition, this shared resource has also banked specimens from patients enrolled in therapeutic vaccination protocols for HPV-associated squamous cancers of the head and neck. All biospecimens are harvested and banked in accordance with the National Cancer Institute�s Best Practice Guidelines for Biorepositories. The core has been expanded to include expert pathologic and immunologic consultation to investigators, including guidance for quantitative digital image analyses-guided molecular studies of tissues. Specimens will be collected under the supervision of pathologists with expertise in gynecologic pathology, in close collaboration with clinical colleagues in these areas. Clinical information for subjects enrolled in our clinical protocols is entered into a password-protected web-based tracking system. This internal web-based system follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A review mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins Cervical Cancer SPORE. Biosamples have been shared with collaborators at academic institutions across the country.