Tissue/Pathology & Immunology Core

该共享资源的目的是收集和分发与宫颈癌和HPV疾病相关的临床注释的人类生物标本，并为约翰霍普金斯大学/阿拉巴马大学/科罗拉多大学/西奈山宫颈癌孢子的研究人员提供病理和免疫学专业知识和组织分析支持。组织核自1998年以来一直存在，并在宫颈癌孢子的支持下扩大。到目前为止，我们已经收集了810例妇科良性组织、2220例肿瘤切除和178例宫颈HPV侵袭前病变的冷冻组织样本。我们还获得了参与CIN2/3方案的245名受试者纵向获得的去角质细胞和外周血标本，以及一次性受试者匹配的良性组织（206例）和632例肿瘤样本的外周血标本。福尔马林固定块可用于几乎所有这些标本。此外，这一共享资源还储存了来自参加hpv相关头颈部鳞状癌治疗性疫苗接种方案的患者的标本。所有的生物标本都是按照国家癌症研究所的生物储存库最佳实践指南收集和储存的。核心已经扩展到包括专家病理和免疫学咨询调查员，包括指导定量数字图像分析，引导组织的分子研究。标本将在具有妇科病理学专业知识的病理学家的监督下收集，并与这些领域的临床同事密切合作。在我们的临床方案中登记的受试者的临床信息被输入一个密码保护的基于网络的跟踪系统。这个基于web的内部系统遵循国家研究委员会的建议，包括用户身份验证、加密、审计跟踪和灾难恢复。一个审查机制已经到位，以优先分配所要求的资源给约翰霍普金斯大学宫颈癌孢子研究中心内外的调查人员。生物样本已与全国各地学术机构的合作者共享。

The purpose of this shared resource is to collect and distribute clinically annotated human biospecimens related to cervical cancer and HPV disease, and to provide pathologic and immunologic expertise and support for tissue-based analyses for investigators in the Johns Hopkins/University of Alabama/University of Colorado/Mount Sinai Cervical Cancer SPORE. The tissue core has been in existence since 1998, and has expanded with the support of the Cervical Cancer SPORE. To date, we have banked frozen tissue samples from 810 benign gynecologic tissues, 2,220 tumor resections, and 178 resections of preinvasive cervical HPV lesions. Longitudinally obtained exfoliated cell and peripheral blood specimens from 245 subjects enrolled in our CIN2/3 protocols have also been obtained, as well as one-time subject-matched peripheral blood samples for benign tissues (206), and from 632 tumor samples. Formalin fixed blocks are available for virtually all of these specimens. In addition, this shared resource has also banked specimens from patients enrolled in therapeutic vaccination protocols for HPV-associated squamous cancers of the head and neck. All biospecimens are harvested and banked in accordance with the National Cancer Institute�s Best Practice Guidelines for Biorepositories. The core has been expanded to include expert pathologic and immunologic consultation to investigators, including guidance for quantitative digital image analyses-guided molecular studies of tissues. Specimens will be collected under the supervision of pathologists with expertise in gynecologic pathology, in close collaboration with clinical colleagues in these areas. Clinical information for subjects enrolled in our clinical protocols is entered into a password-protected web-based tracking system. This internal web-based system follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A review mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins Cervical Cancer SPORE. Biosamples have been shared with collaborators at academic institutions across the country.