Copper Acquisition by Methanotrophs.

甲烷氧化菌对铜的获取。

基本信息

批准号：
9036409
负责人：
Laura Dassama
金额：
$ 5.8万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-01 至 2017-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9036409
关键词：
Active Biological Transport Affinity Alkanesulfonates Anabolism Animal Model Bacteria Binding Biochemical Bioinformatics Biological Process Biosynthetic Proteins Carbon Carbon Dioxide Carrier Proteins Cells Complex Copper Cysteine Disulfides Effectiveness Environment Enzymes Escherichia coli Exhibits Gases Goals Gram-Negative Bacteria Health Homeostasis Human Activities Integral Membrane Protein Investigation Kinetics Lead Literature Membrane Metals Methane Methane Metabolism Pathway Methane hydroxylase Methanol Methylocystis Methylosinus trichosporium Natural Products Nutrient Operon Oxazolone Particulate Phenotype Physiological Post-Translational Protein Processing Proteins Radiation Reaction Research Role Route Source Structure Substrate Specificity Thioamides Threonine Transmembrane Domain United States Vertebral column Work base biophysical techniques cofactor greenhouse gases insight oxidation receptor remediation small molecule

项目摘要

DESCRIPTION (provided by applicant): Methane is the second most prevalent greenhouse gas emitted in the United States as a result of human activity. Because of methane's effectiveness at trapping radiation, it is considered significantly more deleterious to the environment than carbon dioxide. Methanotrophs are gram-negative bacteria that metabolize methane and may provide routes for the remediation of the gas. In the first step of metabolizing methane, methanotrophs use an integral membrane protein called particulate methane monooxygenase (pMMO) to oxidize methane to methanol. pMMO is copper-dependent enzyme, and thus methanotrophs have an elevated requirement for copper (Cu). Under conditions of limiting Cu, some methanotrophs secrete a small natural product-like molecule that binds Cu with high affinity. This molecule, called methanobactin (Mbn), is re- internalized into the cell in its Cu-loaded form to satisfy the cell's copper needs. The proposed research will characterize the transporter implicated in the re-internalization of the Cu-Mbn complex in two different methanotrophs. This work will provide insight into the mechanism by which a key requirement for methane metabolism, the acquisition of Cu for pMMO's cofactor, is fulfilled. Additionally, it will provide insight on methanotrophic bacterial homeostasis.

描述（由申请人提供）：甲烷是由于人类活动而在美国发出的第二大普遍的温室气体。由于甲烷在捕获辐射方面的有效性，因此它被认为比二氧化碳更为有害环境。甲烷营养是革兰氏阴性菌的，可代谢甲烷，可以为补救气体提供途径。在代谢甲烷的第一步中，甲烷营养使用了一种总膜蛋白，称为颗粒甲烷单氧酶（PMMO）将甲烷氧化为甲醇。 PMMO是铜依赖性酶，因此甲烷营养的铜（CU）需求升高。在限制CU的条件下，一些甲烷嗜分泌的小型天然产物样分子与高亲和力结合Cu。该分子称为甲苯甲酸（MBN）其cu负载的形式满足了细胞的铜需求。拟议的研究将表征与两种不同甲基营养物中Cu-MBN复合物重新内部化有关的转运蛋白。这项工作将洞悉甲烷代谢的关键需求，即CU以PMMO的辅因子的收购。此外，它将提供有关甲嗜营养性细菌稳态的见解。