Molecular and Antibody Detection of Zika Virus in Saliva at the Point of Care

护理点唾液中寨卡病毒的分子和抗体检测

• 批准号: 9265276
• 负责人: Haim H Bau
• 金额: $ 24.15万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-09 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9265276
• 关键词: Address; Affinity; Americas; Antibodies; Area; Biological Assay; Blood; Body Fluids; Brazil; Cellular Phone; Centers for Disease Control and Prevention (U.S.); Central America; Centrifugation; Characteristics; Clinic; Communication; Culicidae; Custom; Data; Dengue Virus; Dentists; Detection; Development; Devices; Diagnostic; Diagnostic tests; Disease Outbreaks; Enzyme-Linked Immunosorbent Assay; FDA approved; Fetal Development; Fetus; Flavivirus Infections; Gold; Health; Heating; Home environment; Hybrids; Immunoassay; Individual; Infection; International; Label; Laboratories; Liquid substance; Mediating; Methods; Microcephaly; Microfluidic Microchips; Microfluidics; Modification; Molecular; Monitor; Neurologic; Nucleic Acids; Oral; Panama; Patients; Pennsylvania; Performance; Point-of-Care Systems; Pregnancy; Pregnant Women; Process; Reaction; Reporting; Research; Resources; Reverse Transcriptase Polymerase Chain Reaction; Reverse Transcription; Risk Management; Safety; Saliva; Sampling; Seasons; Serum; Signal Transduction; South America; Specificity; Symptoms; System; Technology; Test Result; Testing; Universities; Urine; Vaccine Therapy; Vaccines; Vascular blood supply; Viral; Viral Antigens; Viremia; Virus Diseases; Visual; Work; Zika Virus; base; cost; cross reactivity; design; improved; instrument; instrumentation; laboratory facility; molecular diagnostics; pandemic disease; pathogen; point of care; point-of-care diagnostics; public health emergency; seroconversion; therapy development; tool; viral detection

ABSTRACT Zika virus (ZIKV) infection with its devastating impact on neurological development of fetuses is already a major health problem in South and Central America, and is likely to impact parts of the USA. Reliable diagnostic tools for ZIKV are vital since ZIKV-infected individuals display either no symptoms or nonspecific symptoms similar to other, more common, viral infections. These diagnostic tools are also essential for management of risks during pregnancy; assessing efficacy of vaccines and therapies; tracking the spread of the infection, tracking ZIKV effects on fetal development; assisting in eradication efforts; and assuring the safety of blood supply. Currently, according to the CDC, no reliable diagnostic tools for ZIKV are available and pipeline technologies are mostly focused on methods suitable for centralized laboratories and inappropriate for resource poor settings and the point of care. This collaborative effort between the University of Pennsylvania and the Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES) in Panama will research and develop a hybrid, inexpensive, point of care system for combined molecular detection of ZIKV and of antibodies against the ZIKV in oral samples. The proposed system can be used in the clinic, doctor and dentist office, and at home with minimal instrumentation and (optionally) without electrical power. To reduce cost, enable quantification, the system utilizes a smartphone for signal detection, processing, and communications. The system, in its proposed form, is suitable for individual diagnostics, but is amenable to concurrent processing of large numbers of samples. The Penn team will develop the assays: reverse transcription isothermal enzymatic amplification (RT-LAMP) of viral nucleic acids with visual detection of amplicons and an immunoassay to monitor separately antibodies interactions with ZIKV and dengue virus (DENV) antigens to improve assay specificity; and the necessary hardware. ICGES will verify system's performance and ease of use with samples of ZIKV patients in Panama.

抽象的 寨卡病毒（ZIKV）感染对胎儿神经发育具有毁灭性影响，已成为一种严重的疾病。 南美洲和中美洲的主要健康问题，并可能影响美国部分地区。可靠的 ZIKV 诊断工具至关重要，因为 ZIKV 感染者要么没有症状，要么没有特异性 症状与其他更常见的病毒感染相似。这些诊断工具对于 怀孕期间的风险管理；评估疫苗和疗法的功效；追踪传播情况 感染，追踪 ZIKV 对胎儿发育的影响；协助根除工作；并确保 血液供应安全。据 CDC 称，目前还没有可靠的 ZIKV 诊断工具，并且 管道技术主要集中于适合集中实验室的方法，而不适合于 资源匮乏的环境和护理点。宾夕法尼亚大学之间的合作努力 巴拿马的 Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES) 将研究和 开发一种混合、廉价的护理点系统，用于 ZIKV 和 ZIKV 的联合分子检测 口腔样本中存在抗 ZIKV 的抗体。所提出的系统可用于诊所、医生和 牙医诊所和家里，只需最少的仪器，并且（可选）没有电源。减少 成本，启用量化，该系统利用智能手机进行信号检测、处理和 通讯。该系统以其提议的形式适用于个人诊断，但也适用于 并发处理大量样本。宾夕法尼亚大学团队将开发检测方法：反向 病毒核酸转录等温酶扩增 (RT-LAMP)，可目视检测 扩增子和免疫测定可分别监测抗体与 ZIKV 和登革热病毒的相互作用 (DENV) 抗原以提高检测特异性；以及必要的硬件。 ICGES 将验证系统的 巴拿马 ZIKV 患者样本的性能和易用性。