Rapid, High Yield, Single-Tube Nucleic Acid Extraction at Low Temperature using Lateral Ultrasonic Wave Transducer

 描述(申请人提供)：基因分析是“个性化医学”背后的驱动力，并已成为推动生物学、医学和行为生物学所有领域的重要工具。随着医学遗传样本分析技术的不断发展，最终将从将样本送到实验室的旧模式转变为护理点式样本分析。Microsonic Systems Inc.计划开发一种用于核酸分析的快速便携式样品制备设备，该设备使用我们的散装横向超声(BLU)波换能器进行医疗诊断。该仪器开发的关键将是能够在冷状态下在单个试管中快速执行多步骤的核酸样品制备(&lt；25 C.)温度。第一阶段的具体目标包括：a)使用正常的冷冻人体肌肉和皮肤组织来优化BLU能量参数和核酸提取(NAE)协议；以及b)评估和验证优化的BLU能量核酸提取(NAE)协议，覆盖来自多种人体组织类型(如乳房、肾脏、前列腺、肝脏和甲状腺)的正常和肿瘤样本。开发一种护理点便携式仪器(第二阶段)，以帮助检测/治疗与这些组织群相关的肿瘤学，是卫生保健行业潜在的范式转变概念。

 DESCRIPTION (provided by applicant): Genetic analysis is a driving force behind "Personalized Medicine" and has become an essential tool in advancing all areas of biology, medicine, and behavioral biology. As medical genetic sample analysis technology continues to develop there will eventually be a shift from the old paradigm of sending the sample out to the lab, to point-of-care sample analysis. Microsonic Systems Inc. proposes to develop a rapid portable sample prep device for nucleic acid analysis using our Bulk Lateral Ultrasonic (BLU) Wave Transducer for point-of-care diagnostics. Key to this instrument development will be the ability to rapidly perform multiple steps of nucleic acid sample prep in a single tube at cold (<25 C.) temperatures. Phase I specific aims include: A) optimization of the BLU energy parameters and nucleic acid extraction (NAE) protocol using normal frozen human muscle and skin tissues; and B) to evaluate and validate the optimized BLU energy nucleic acid extraction (NAE) protocol across normal and tumor samples from multiple human tissue types (e.g., breast, kidney, prostate, liver and thyroid). Development of a point-of-care portable instrument (Phase II) to aid in detection/treatment of oncology associated with these tissue groups is a potential paradigm shifting concept for the health care industry.