Rapid, High Yield, Single-Tube Nucleic Acid Extraction at Low Temperature using Lateral Ultrasonic Wave Transducer

使用横向超声波换能器在低温下快速、高产、单管核酸提取

• 批准号: 9046543
• 负责人: Poonam Sansanwal
• 金额: $ 15万
• 依托单位: MICROSONIC SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9046543
• 关键词: Area; Behavioral Medicine; Biological Assay; Biological Markers; Biology; Breast; Chronic Kidney Failure; Connective Tissue; Cytolysis; DNA; Detection; Development; Device or Instrument Development; Devices; Diagnosis; Evaluation; Fluorescence; Freezing; Goals; Health Care Costs; Healthcare Industry; Histocompatibility Testing; Hospital Departments; Human; Kidney; Lateral; Liver; Mediating; Medical Genetics; Molecular Diagnosis; Muscle; Myocardium; Nucleic Acids; Patients; Phase; Polymerase Chain Reaction; Process; Prostate; Protocols documentation; RNA; Sampling; Skeletal Muscle; Skin; Skin Tissue; Striated Muscles; System; Techniques; Technology; Temperature; Testing; Therapeutic; Thyroid Diseases; Thyroid Gland; Time; Tissue Sample; Tissues; Transducers; Tube; Tumor Tissue; Ultrasonic wave; Ultrasonics; Ultrasonography; Workload; cancer biomarkers; cold temperature; driving force; genetic analysis; human tissue; improved; instrument; magnetic beads; nucleic acid detection; oncology; personalized medicine; point of care; point-of-care diagnostics; public health relevance; qubit; tool; tumor; tumor heterogeneity

 DESCRIPTION (provided by applicant): Genetic analysis is a driving force behind "Personalized Medicine" and has become an essential tool in advancing all areas of biology, medicine, and behavioral biology. As medical genetic sample analysis technology continues to develop there will eventually be a shift from the old paradigm of sending the sample out to the lab, to point-of-care sample analysis. Microsonic Systems Inc. proposes to develop a rapid portable sample prep device for nucleic acid analysis using our Bulk Lateral Ultrasonic (BLU) Wave Transducer for point-of-care diagnostics. Key to this instrument development will be the ability to rapidly perform multiple steps of nucleic acid sample prep in a single tube at cold (<25 C.) temperatures. Phase I specific aims include: A) optimization of the BLU energy parameters and nucleic acid extraction (NAE) protocol using normal frozen human muscle and skin tissues; and B) to evaluate and validate the optimized BLU energy nucleic acid extraction (NAE) protocol across normal and tumor samples from multiple human tissue types (e.g., breast, kidney, prostate, liver and thyroid). Development of a point-of-care portable instrument (Phase II) to aid in detection/treatment of oncology associated with these tissue groups is a potential paradigm shifting concept for the health care industry.

 描述（由申请人提供）：遗传分析是“个性化医疗”背后的驱动力，并已成为推进生物学，医学和行为生物学所有领域的重要工具。随着医学遗传样本分析技术的不断发展，最终将从将样本送到实验室的旧模式转变为即时样本分析。Microsonic Systems Inc.建议开发一种快速便携式样品制备设备，用于核酸分析，使用我们的Bulk Lateral Ultrasonic（BLU）Wave Transducer进行床旁诊断。该仪器开发的关键将是能够在低温（<25 ℃）下在单个管中快速执行核酸样品制备的多个步骤 C.）温度第一阶段的具体目标包括：A）使用正常冷冻人肌肉和皮肤组织优化BLU能量参数和核酸提取（NAE）方案;和B）在来自多种人组织类型（例如，乳腺、肾脏、前列腺、肝脏和甲状腺）。开发一种即时便携式仪器（第二阶段），以帮助检测/治疗与这些组织组相关的肿瘤，是医疗保健行业潜在的范式转变概念。