Investigating the roles of lncRNAs in cancer and development
研究 lncRNA 在癌症和发育中的作用
基本信息
- 批准号:9062295
- 负责人:
- 金额:$ 42.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-07-01 至 2019-05-31
- 项目状态:已结题
- 来源:
- 关键词:AgeApplications GrantsB-Cell LymphomasB-LymphocytesBiologicalBiological ModelsBiological ProcessBiologyCell Differentiation processCodeCommunitiesDataDevelopmentDiseaseDoxycyclineEctopic ExpressionEmbryonic DevelopmentEnsureEpigenetic ProcessGenerationsGeneticGenetic studyGenetically Engineered MouseGoalsHealthHematopoiesisHumanHuman GenomeIn VitroIndividualInvestigationLeadLymphocyteLymphomagenesisMalignant NeoplasmsModalityMolecularMusOncogenicOrthologous GenePathogenesisPhasePhysiologicalPlayPrognostic MarkerPropertyProteinsPublic HealthRNA interference screenRegulationResearch ProposalsRoleSystemTestingTherapeuticTransgenic OrganismsUntranslated RNAWorkagedbasedesigndiagnostic biomarkerdifferential expressiongain of functionhigh throughput screeningin vivoinnovationinsightinterdisciplinary approachloss of functionmammalian genomemouse modelnovelnovel diagnosticsnovel therapeutic interventionresearch studyscreeningtranscriptometranscriptome sequencingtumortumor progressiontumorigenesis
项目摘要
DESCRIPTION (provided by applicant): Mammalian genomes are pervasively transcribed, but only a fraction of the transcribed RNAs appears to encode for proteins. Among the non-coding component of the transcriptome, long intergenic non-coding RNAs (lincRNAs) have recently been the subject of intense investigation due to their potential roles in cell differentiation, development, and disease. Although several lincRNAs have been proposed to play a role in the pathogenesis of human cancers, definitive experimental evidence is still missing and our understanding of the precise biological functions and modality of action of the vast majority of them is minimal or absent. Here we propose to investigate the potential roles of lincRNAs in the pathogenesis of Myc-driven B cell lymphomas. Our central hypothesis is that a subset of lincRNAs possesses oncogenic or tumor suppressive properties and that these lincRNAs contribute to lymphomagenesis. To test this hypothesis we are proposing a multidisciplinary approach that consists in a first high-throughput phase in which we aim to identify lincRNAs that are de-regulated during Myc-driven lymphomagenesis. Next, this set of lincRNAs will be subjected to in vitro and in vivo functional screens to identify those that can accelerate or impai tumor progression. Finally, the most promising candidates will be investigated through loss of function and gain of function experiments in genetically engineered mice. Our ultimate goals are to define the roles of these lincRNAs in tumorigenesis, determine their physiologic functions, and identify their mechanisms of action. We predict that the successful completion of the experiments described in this proposal will provide important insights into the biology of this important class of non-coding RNAs and will define their potential roles in tumorigenesis.
描述(由申请人提供):哺乳动物基因组普遍转录,但只有一小部分转录的rna似乎编码蛋白质。在转录组的非编码成分中,长基因间非编码rna (lincRNAs)由于其在细胞分化、发育和疾病中的潜在作用,近年来一直是研究的重点。虽然一些lincrna已经被提出在人类癌症的发病机制中发挥作用,但明确的实验证据仍然缺失,我们对绝大多数lincrna的精确生物学功能和作用方式的理解很少或不存在。在这里,我们建议研究lincRNAs在myc驱动的B细胞淋巴瘤发病机制中的潜在作用。我们的中心假设是lincRNAs的一个子集具有致癌或肿瘤抑制特性,并且这些lincRNAs有助于淋巴瘤的发生。为了验证这一假设,我们提出了一种多学科方法,包括在第一个高通量阶段,我们的目标是确定在myc驱动的淋巴瘤形成过程中去调控的lincrna。接下来,这组lincrna将在体外和体内进行功能筛选,以确定那些可以加速或影响肿瘤进展的lincrna。最后,将通过基因工程小鼠的功能丧失和功能获得实验来研究最有希望的候选者。我们的最终目标是确定这些lincrna在肿瘤发生中的作用,确定它们的生理功能,并确定它们的作用机制。我们预测,本提案中描述的实验的成功完成将为这类重要的非编码rna的生物学提供重要的见解,并将确定它们在肿瘤发生中的潜在作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Andrea Ventura其他文献
Andrea Ventura的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Andrea Ventura', 18)}}的其他基金
Investigating the roles of oncogenic extrachromosomal circular DNAs in cancer
研究致癌染色体外环状 DNA 在癌症中的作用
- 批准号:
10718423 - 财政年份:2023
- 资助金额:
$ 42.73万 - 项目类别:
Investigating microRNA Function in Homeostasis, Regeneration and Cancer
研究 microRNA 在稳态、再生和癌症中的功能
- 批准号:
10678921 - 财政年份:2020
- 资助金额:
$ 42.73万 - 项目类别:
Investigating microRNA Function in Homeostasis, Regeneration and Cancer
研究 microRNA 在稳态、再生和癌症中的功能
- 批准号:
10242920 - 财政年份:2020
- 资助金额:
$ 42.73万 - 项目类别:
Investigating microRNA Function in Homeostasis, Regeneration and Cancer
研究 microRNA 在稳态、再生和癌症中的功能
- 批准号:
10407066 - 财政年份:2020
- 资助金额:
$ 42.73万 - 项目类别:
Investigating the roles of lncRNAs in cancer and development
研究 lncRNA 在癌症和发育中的作用
- 批准号:
8882352 - 财政年份:2014
- 资助金额:
$ 42.73万 - 项目类别:
Investigating the roles of lncRNAs in cancer and development
研究 lncRNA 在癌症和发育中的作用
- 批准号:
8760845 - 财政年份:2014
- 资助金额:
$ 42.73万 - 项目类别:
Investigating the roles of lncRNAs in cancer and development
研究 lncRNA 在癌症和发育中的作用
- 批准号:
9266375 - 财政年份:2014
- 资助金额:
$ 42.73万 - 项目类别:
Investigating the functions of the miR-17~92 family of oncogenic microRNA cluster
致癌microRNA簇miR-17~92家族功能的研究
- 批准号:
8034401 - 财政年份:2010
- 资助金额:
$ 42.73万 - 项目类别:
Investigating the functions of the miR-17~92 family of oncogenic microRNA clusters
研究致癌 microRNA 簇的 miR-17~92 家族的功能
- 批准号:
9247341 - 财政年份:2010
- 资助金额:
$ 42.73万 - 项目类别:
Investigating the functions of the miR-17~92 family of oncogenic microRNA clusters
研究致癌 microRNA 簇的 miR-17~92 家族的功能
- 批准号:
10064603 - 财政年份:2010
- 资助金额:
$ 42.73万 - 项目类别: