ShEEP Request for Nikon Eclipse Ni Confocal Scanning Microscope

ShEEP 请求尼康 Eclipse Ni 共焦扫描显微镜

• 批准号: 9211715
• 负责人: HOLLY VAN REMMEN
• 金额: --
• 依托单位: OKLAHOMA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9211715
• 关键词: Alzheimer' s Disease; Amyotrophic Lateral Sclerosis; Antibodies; Area; Binding; Biomedical Research; Blood Platelets; Brain; Brain Mapping; Calcium Channel; Cells; Cerebrovascular Disorders; Cities; Colorectal Cancer; Complication; Confocal Microscopy; DNA Damage; Development; Disease; Epilepsy; Equipment; Event; Fluorescence; Foundations; Funding; Genomic Instability; Grant; Health; Health Sciences; Healthcare Systems; Holly; Housing; Image; Intestines; Lasers; Lighting; Maintenance; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Medical Research; Medical center; Microscope; Minor; Myeloid Cells; Neuromuscular Junction; Oklahoma; Optics; Osteoclasts; Peptides; Proteins; Reporting; Research; Research Personnel; Research Project Grants; Resolution; Role; Sampling; Satiation; Scanning; Scientist; Signal Transduction; Site; Sjogren' s Syndrome; Stem cells; Surveys; System; Three-Dimensional Image; Time; Tissues; Universities; Veterans; Visceral pain; base; career; design; fluorescence imaging; frailty; imaging system; instrument; laboratory facility; live cell imaging; microscopic imaging; nanocrystal; novel; prevent; research facility; sarcopenia; user-friendly

We are requesting funds to purchase a Nikon Eclipse Ni Confocal Laser-Scanning Microscope. Confocal microscopy has become a mainstay for biomedical research allowing researchers to image live cells and tissues, follow dynamic events between or within cells, and to make 3D images of cells/tissues. Currently, the research facility at the Oklahoma City VA Health Care System (OKCVA HCS) has no fluorescent or confocal microscopes. VA investigators that require fluorescent/confocal imaging for their VA-funded research have to use confocal microscopes at the Imaging Cores at either the University of Oklahoma Health Sciences Center (OUHSC) or the Oklahoma Medical Research Foundation (OMRF). The rational for purchasing Nikon Eclipse Ni Confocal Laser-Scanning Microscope is: (1) Almost all the funded VA investigators as well as the VA investigators with pending or revised applications that will be submitted in March will need to use a fluorescent/confocal microscope in their future research, i.e., there is a great need for this instrument at the OKCVA HCS; (2) It is inconvenient and time-consuming to take samples from the OKCVA HCS to the Imaging Cores at either OUHSC or OMRF to be analyzed; and (3) Imaging live cells requires that VA investigators find tissue facilities that they can use in the building that houses the fluorescent/confocal microscopes at OUHSC or OMRF. The Nikon Eclipse Ni confocal microscope allows multi-fluorescence imaging of a variety of tissues with high resolution and contrast. The microscope uses novel optical objectives coated with Nano crystal coat for brighter and high contrast microscopic images. Its design enables simultaneous multi-channel imaging using laser illumination of the fluorescent proteins. The system uses a new LU-N4 laser unit equipped with four lasers (405nm, 488nm, 561nm, 640nm) in a space-saving mode. This newly developed laser combiner prevents alignment shift even after long-term use and allows the laser power to be controlled and modulated in a “user-friendly” manner and dramatically reduces the need to constantly adjust the lasers on the microscope, which was a major complication that required a significant amount of maintenance in older confocal microscopes. A survey of the VA researchers at OKCVA HCS showed over 80% of the researchers have used the fluorescent confocal microscopes at the Imaging Cores at either OUHSC or OMRF for their VA funded research over the past 3 or 4 years. In addition, the VA researchers unanimously listed a fluorescent confocal microscope at the top of their list of the major piece of equipment that was needed at the OKCVA HCS. The Nikon Eclipse Ni confocal microscope will be used by seven major users who have VA Merit grants and three minor users who have VA Merit grants pending.

我们正在申请资金购买Nikon Eclipse Ni共聚焦激光扫描显微镜。共焦 显微镜已经成为生物医学研究的支柱，使研究人员能够对活细胞进行成像， 组织，跟踪细胞之间或细胞内的动态事件，并制作细胞/组织的3D图像。目前 俄克拉荷马州城市VA卫生保健系统（OKCVA HCS）的研究机构没有荧光或 共焦显微镜VA研究者需要荧光/共聚焦成像进行VA资助 研究必须在俄克拉荷马州卫生大学的成像中心使用共聚焦显微镜 科学中心（OUHSC）或俄克拉荷马州医学研究基金会（OMRF）。的合理性 购买Nikon Eclipse Ni共聚焦激光扫描显微镜的原因是：（1）几乎所有资助的VA 调查人员以及VA调查人员，他们将在 March将需要在他们未来的研究中使用荧光/共聚焦显微镜，即，非常需要 （2）在OKCVA HCS中使用该仪器;（3）从OKCVA HCS中采集样品不方便且耗时 OKCVA HCS到OUHSC或OMRF的成像核心进行分析;以及（3）活细胞成像 要求VA调查人员找到他们可以在容纳 OUHSC或OMRF的荧光/共聚焦显微镜。 Nikon Eclipse Ni共聚焦显微镜允许多种组织的多荧光成像， 高分辨率和对比度。该显微镜采用新型光学物镜，并涂有纳米晶体涂层， 更明亮和高对比度的显微图像。它的设计可以同时进行多通道成像， 荧光蛋白的激光照射。该系统使用一个新的LU-N4激光单元，配备四个 激光器（405 nm，488 nm，561 nm，640 nm），以节省空间的模式。这个新开发的激光组合器 即使在长期使用后也能防止对准偏移，并允许控制和调制激光功率， 一种“用户友好”的方式，并大大减少了需要不断调整显微镜上的激光， 这是一个主要的并发症，需要大量的维护，在旧的共聚焦 显微镜. 一项对OKCVA HCS的VA研究人员的调查显示，超过80%的研究人员使用了 荧光共聚焦显微镜在成像核心无论是在OUHSC或OMRF为他们的VA资助 在过去的三、四年里，此外，弗吉尼亚州的研究人员一致列出了一个荧光共聚焦 显微镜在他们的列表的顶部的主要设备是需要在OKCVA HCS。的 尼康Eclipse Ni共聚焦显微镜将由七个拥有VA Merit赠款的主要用户和三个 未成年人用户谁有VA优异奖助学赠款。