Lipid-protein interactions in bacterial reaction centers reconstituted into liposomes

重组为脂质体的细菌反应中心中的脂质-蛋白质相互作用

• 批准号: 326522-2006
• 负责人: Kalman, Laszlo
• 金额: $ 2.62万
• 依托单位: Concordia University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=331018
• 关键词: Lipid; protein; interactions; bacterial; reaction

The aim of the research is to contribute toward an understanding how lipid-protein interactions in membrane proteins modify the properties of molecules that undergo oxidation-reduction reactions. These interactions are crucial for basic processes, such as electron and proton transfer reactions. The model systems are membrane-bound photosynthetic reaction centers isolated from purple bacteria. The experimental approach is focused on systematic reconstitution of the reaction center protein into different artificial membrane systems:liposomes and mixed lipid-detergent micelles.  The alterations in the surrounding lipid environment of the reaction center protein should reflect in changes of the parameters of the electron and proton transfer reactions involving the redox cofactors and nearby protonatable amino acid side chains. The ultimate goal of the project is to assemble a proteoliposome system where the electron and proton transfer parameters are optimized and closely resemble the native system. The results of these model systems can provide basic information important to better understanding the processes fundamental to energy conversion.

这项研究的目的是为了帮助理解膜蛋白中的脂-蛋白相互作用如何改变经历氧化还原反应的分子的性质。这些相互作用对电子和质子转移反应等基本过程至关重要。模型系统是从紫色细菌中分离出来的膜结合的光合作用反应中心。实验方法侧重于系统地将反应中心蛋白质重组为不同的人工膜系统：脂质体和脂类-洗涤剂混合胶束。反应中心蛋白质周围脂环境的变化应反映在涉及氧化还原辅因子和附近可质子化氨基酸侧链的电子和质子转移反应参数的变化。该项目的最终目标是组装一种蛋白质脂质体系统，其中电子和质子转移参数得到优化，并与天然系统非常相似。这些模型系统的结果可以提供对更好地理解能量转换的基本过程非常重要的基本信息。