Rapid profiling of whole samples by mass spectrometry

通过质谱法快速分析整个样品

• 批准号: 1952-2006
• 负责人: Helleur, Robert
• 金额: $ 1.68万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=336233
• 关键词: Rapid; profiling; whole; samples; mass

The objective of this research is to develop new mass spectrometric techniques for the rapid profiling of whole samples by exploring novel approaches to sample introduction. In particular, we are developing innovative methodologies in 1) thermally assisted in-situ derivatization coupled to gas chromotography/mass spectrometry, 2) thin layer chromatography interfaced to mass spectrometry  and 3) matrix-assisted laser desorption time of flight mass spectrometry for small molecules. These methods will be developed for applications in the emerging field of metabolomics (i.e., chemical profiling of all metabolites in a sample), for identifying useful biomarkers for chemotaxonomy and for environmental air monitoring of mold and bacteria. Results will enhance the analytical capability of mass spectrometry to more rapidly profile and screen the chemical composition of whole samples and complement existing mass spectrometric and chromatographic techniques. These methods will shorten analysis time and reduce the use of hazardous extraction solvents (green chemistry).

本研究的目的是开发新的质谱技术，通过探索新的样品引入方法快速分析整个样品。特别是，我们正在开发创新的方法：1）热辅助原位衍生与气相色谱/质谱联用，2）薄层色谱与质谱联用，3）小分子基质辅助激光解吸飞行时间质谱。这些方法将被开发用于新兴的代谢组学领域（即，样品中所有代谢物的化学特征分析），用于鉴定化学分类学的有用生物标志物以及用于霉菌和细菌的环境空气监测。研究结果将提高质谱分析能力，更迅速地分析和筛选整个样品的化学成分，并补充现有的质谱和色谱技术。这些方法将缩短分析时间并减少危险提取溶剂的使用（绿色化学）。