Plate reader - Bamfield marine sciences centre

读板器 - 班菲尔德海洋科学中心

• 批准号: 360472-2008
• 负责人: Palmer, Richard
• 金额: $ 5.14万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364561
• 关键词: Plate; reader; Bamfield; marine; sciences

The Bamfield Marine Sciences Centre (BMSC) supports the research of visiting scientists from over 40 Canadian, U.S. and international institutions who study a wide range of subjects, including marine aspects of comparative physiology, molecular and cell biology, ecology and evolutionary biology, neuroscience, invasive species biology, developmental and reproductive biology, and conservation biology.  Laboratory biologists who utilize BMSC face an acute problem:  it is impractical or expensive to ship specialized equipment from their home laboratories to BMSC.  Furthermore, in addition to depriving research personnel in their home laboratories of its use, some past attempts to ship equipment to BMSC have resulted in irreparable damage or expensive repair bills.  Increasingly, BMSC is becoming a base to a broad range of Canadian comparative physiologists because of the potent mixture of research facilities, high-quality running seawater, and easy access to a great variety of research animals.  The research activities of this growing and highly collaborative group of researchers are increasingly constrained by lack of access to automated equipment that most have in their home laboratories.  A requirement therefore exists for an automated microplate reader/washer/fluorescence system:  1) to support a wide variety of metabolite concentration and enzyme activity assays, 2) to facilitate much more rapid and efficient ELISA assays via an automated plate washing system, and 3) to measure excitation/emission wavelengths using several varieties of fluorophores.  The ability to do such assays will greatly aid researchers who study a wide range of subjects including:  effects of feeding, exercise and stress on fish metabolic and respiratory physiology, tolerance of ammonia and air-exposure in intertidal fish, evolution of blood compartment acid-base regulation and ionic regulation in primitive vertebrates and in euryhaline fish, social and reproductive physiology, and metal toxicity in teleosts, elasmobranchs, and assorted invertebrates.

班菲尔德海洋科学中心(BMSC)支持来自40多个加拿大、美国和国际机构的来访科学家的研究，他们研究的学科范围很广，包括比较生理学、分子和细胞生物学、生态学和进化生物学、神经科学、入侵物种生物学、发育和生殖生物学以及保护生物学的海洋方面。使用BMSC的实验室生物学家面临着一个尖锐的问题：从他们的家庭实验室向BMSC运送专门的设备是不切实际的或昂贵的。此外，除了剥夺他们家庭实验室的研究人员的使用之外，过去向BMSC运送设备的一些尝试导致了无法弥补的损坏或昂贵的维修费。BMSC正越来越多地成为广泛的加拿大比较生理学家的基地，因为研究设施、高质量的流动海水和容易获得各种研究动物的强有力的组合。这一日益壮大和高度合作的研究人员群体的研究活动越来越受到缺乏访问大多数家庭实验室中的自动化设备的限制。因此，存在对自动微量平板阅读器/洗涤器/荧光系统的需求：以支持各种代谢物浓度和酶活性分析，2)通过自动洗板系统促进更快速和有效的ELISA法分析，以及3)使用几种荧光团来测量激发/发射波长。进行这种分析的能力将极大地帮助研究广泛课题的研究人员，这些课题包括：摄食、运动和应激对鱼类代谢和呼吸生理学的影响；潮间带鱼类对氨和空气暴露的耐受性；原始脊椎动物和泛盐生鱼类血液隔室酸碱调节和离子调节的演变；社会和生殖生理学；以及硬骨鱼、弹枝和各种无脊椎动物中的金属毒性。