Hormonal regulation of mineral metabolism in fishes and invertebrates

目前的建议旨在继续我们的长期研究计划，即如何通过多肽激素STC-1(STC-1)调节鱼类和水蚤中的钙和磷稳态。目的I.鲑鱼鳃中富含线粒体的氯化细胞具有高水平的STC-1受体。我们将探索STC-1靶向氯化细胞线粒体的可能性，作为STC-1抑制鳃钙转运的作用机制的一部分。目标II：鲑鱼卵巢中的早期卵母细胞产生一种变种形式的STC-1，它似乎以自分泌或内分泌循环的方式靶向回到这些卵母细胞。我们试图确定激素靶向的亚细胞室，并提纯STC-1的新变种，用于化学分析和生物测定。目的#3.水蚤具有STC相关蛋白和受体，调节皮肤对钙的吸收。我们将研究钙等因素对这一运输过程的调节。我们还将确定水蚤的钙吸收是否会随着时间的推移(几周)而周期性变化，就像鱼类一样。我们还将提纯两种不同的水蚤形式的STC，然后对其结构进行表征，并在生物学研究中对其进行测试。

The current proposal seeks to continue our long term program of studies on how calcium and phosphate homeostasis are regulated in fish and aquatic leeches by the peptide hormone, stanniocalcin-1 (STC-1).Objective I. The mitochondria-rich, chloride cells in trout gill have high levels of STC-1 receptors. We will explore the possibility that STC-1 is targeted to chloride cell mitochondria as part of the mechanism of action by which STC-1 inhibits gill calcium transport.  Objective II. Early oocytes in trout ovary produce a variant form of STC-1 that appears to be targeted back to these oocytes in an autocrine or intracrine loop. We seek to identify the sub-cellular compartment targeted by the hormone and to purify the  variant form of STC-1 for chemical analysis and bioassay.Objective #3. Leeches have STC-related proteins and receptors that regulate calcium uptake by the skin. We will study the regulation of this transport process by factors such as calcium. We will also determine if calcium uptake in leeches cyclical over time (weeks), as it is in fishes. We will also purify two different leech forms of STC, characterize then structurally and test them in biological studies.