Upgrade for delta vision imaging system

生物体的形态和功能是由精确控制的细胞分裂、分化和死亡决定的。许多实验室正在进行的研究表明，直到最近才被认为是大多数细胞的退化细胞器的初级纤毛是决定单个细胞命运的信号整合的场所。Quarmby致力于了解纤毛如何调节细胞分裂，Leroux实验室研究纤毛发生和纤毛的组成。这两个研究小组的发现都影响了我们对一个被称为纤毛病的人类疾病家族的理解。该DeltaVision成像站（DV），这是在2002年购买了非常大的赠款从CFI新机会计划和BC知识发展基金，是在SFU纤毛研究的基石。DV是一种高端集成落射荧光显微镜系统，包括一个独特的照明系统，提供了一个均匀的光源，能够快速改变激发和发射滤光片，一个载物台，可以控制非常高的和可重复的运动在三维，一个显微镜与一流的光学，和一个集成的工作站，控制光源，载物台，光学器件和图像捕获由冷却的电荷耦合器件照相机。通过去卷积去除失焦光。有了这个系统，我们获得了令人惊叹的图像，并取得了重大发现。经过五年的大量使用，这台设备已显老化。有些部件已经磨损，有些部件已经过时。DV对于我们持续生产高质量的细胞生物学研究至关重要，绝对需要升级以保持其正常工作。

The form and function of organisms is determined by precisely controlled cell division, differentiation and death. Ongoing research in numerous laboratories is revealing that the primary cilium, until recently considered a vestigial organelle for most cells, is the site of the integration of signals that determine the fate of individual cells. The Quarmby is concerned with understanding how the cilium regulates cell division and the Leroux lab studies ciliogenesis and the composition of cilia. Both groups have made discoveries that impact our understanding of a family of human diseases known as ciliopathies. The DeltaVision Imaging station (DV), which was purchased in 2002 with very large grants from the CFI New Opportunities program and from the BC Knowledge Development fund, is a cornerstone of ciliary research at SFU. The DV is a high-end integrated epi-fluorescence microscopy system that includes a unique illumination system which provides an even light source with capability of rapid changes in excitation and emission filters, a stage that can be controlled with very high and repeatable motion in three dimensions, a microscope with superb optics, and an integrated workstation that controls the light source, the stage, the optics and image capture by a cooled charge-coupled device camera. Out-of-focus light is removed by deconvolution. With this system we have achieved stunning images and made significant discoveries. After five years of extremely heavy use, the equipment is showing its age. Some parts are worn and others are obsolete. The DV is essential for our continued production of high quality cell biological research and the upgrade is absolutely required to keep it in working order.