Storage and processing equipment

储存及加工设备

• 批准号: 358984-2008
• 负责人: Bielajew, Catherine
• 金额: $ 1.53万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=366102
• 关键词: Storage; processing; equipment

This request is for funding to purchase needed equipment for ulta-low temperature storage and processing of blood and tissue samples for biochemical markers related to hedonic, endocrine, and immune status in our animal models. We use these markers in conjunction with our battery of behavioural and physiological indices to evaluate the influence of reward, whether generated by electrical stimulation or via an enriched environment in male and female rats. We are interested in both the cognitive and emotional consequences of reward experiences in animals that have been exposed to stressors and follow them over the life span. To date, I have depended on the generosity of my colleagues to store and process my expanding collection of tissue and blood samples. The productivity and efficiency of my research program and contribution to student training would be greatly enhanced if in-house analyses such as ELISA could be conducted on tissue stored in a reliable temperature controlled environment. Hence, this is a request for a freezer, plate reader, and incubator/shaker combination.

这项申请是为了购买所需的设备，用于血液和组织样本的超低温存储和处理，以获得与我们动物模型的享乐、内分泌和免疫状态相关的生化标记物。我们将这些标记与我们的一系列行为和生理指标结合使用，以评估奖励的影响，无论是由电刺激产生的，还是通过丰富的环境对雄性和雌性大鼠产生的影响。我们感兴趣的是，在动物暴露在压力源下并在整个生命周期中跟随它们的奖励经历的认知和情感后果。到目前为止，我一直依靠同事的慷慨来存储和处理我不断扩大的组织和血液样本收集。如果能对储存在可靠温控环境中的组织进行内部分析，如ELISA，我的研究计划的生产力和效率以及对学生培训的贡献将大大提高。因此，这是对冷冻箱、平板阅读器和培养箱/摇床组合的请求。