Functional molecular films at charged interfaces

带电界面的功能分子膜

• 批准号: 694-2006
• 负责人: Lipkowski, Jacek
• 金额: $ 8.12万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=388325
• 关键词: Functional; molecular; films; charged; interfaces

The objective of our research is to build functional model membranes at an electrode surface that could be used to study the mechanism of voltage gated phenomena such as transport through ion channels and to study the effect of various drugs on the function of the ion channels.  To achieve these goals we will employ electrochemical methods, scanning probe microscopies such as scanning tunneling microscopy and atomic force microscopy, in situ refelection  spectroscopies in the infrared, ultraviolet and visible range of radiation and neutron reflectivity technique. The metal electrode surface, covered by a film of surfactants or phospholipids, can be charged and electric fields on the order of 108V/m can be applied to these supported films. These fields have comparable magnitude to the fields acting on biological membranes. The field may be conveniently used to manipulate organic molecules within the membrane.  IR reflection absorption spectroscopy can then be used to determine the orientation and conformation of the bio-molecule in the membrane and their changes in response to the applied field.  Neutron reflectivity and surface plasmon resonance (SPR) measurements will provide information about the thickness of the bilayer and the water content in the bilayer.  Our research has always been driven by the development of new methods.  To keep up with this tradition we are planning to develop a technique allowing combined patch clamp and IR spectroscopic studies of the mechanism and kinetics of voltage gated ion channel opening.  Our studies provide basic knowledge that is essential to control the stability of sensors and biosensors and for the use of supported bilayers in biomimetic research.

本研究的目的是在电极表面构建功能模型膜，用于研究电压门控现象的机制，如通过离子通道的传输，并研究各种药物对离子通道功能的影响。为了实现这些目标，我们将采用电化学方法，扫描探针显微镜，如扫描隧道显微镜和原子力显微镜，在红外、紫外和可见辐射范围内的原位反射光谱和中子反射技术。被表面活性剂或磷脂膜覆盖的金属电极表面可以带电，并且可以向这些支撑膜施加108 V/m量级的电场。这些场具有与作用于生物膜的场相当的大小。利用该场可以方便地操纵膜内的有机分子，利用红外反射吸收光谱可以确定生物分子在膜内的取向和构象，以及它们随外加场的变化。中子反射率和表面等离子体共振（SPR）测量将提供有关双层厚度和双层中含水量的信息。我们的研究一直受到新方法开发的推动。为了跟上这一趋势，传统上，我们正计划开发一种技术，允许结合膜片钳和IR光谱研究电压门控离子通道开放的机制和动力学。我们的研究提供了基本的知识，是必不可少的控制传感器和生物传感器的稳定性和使用支持的双层仿生研究。