Stress- and xenobiotic-induced changes in early fish embryo gene expression patterns as biomarkers of development and growth in juveniles

应激和外源性诱导的早期鱼胚胎基因表达模式的变化作为幼鱼发育和生长的生物标志物

基本信息

  • 批准号:
    6974-2008
  • 负责人:
  • 金额:
    $ 3.13万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2008
  • 资助国家:
    加拿大
  • 起止时间:
    2008-01-01 至 2009-12-31
  • 项目状态:
    已结题

项目摘要

The cytoplasm of the vertebrate oocyte plays a key role in regulating or modifying early gene expression by an embryo that develops from that oocyte, and can influence the phenotype of post-embryonic stages. Despite decades of intensive research in mammals leading to the development of reproductive biotechnologies such as in vitro fertilization (IVF) the epigenetic influences of the ooplasm factors are still poorly understood; embryo deformity rates in IVF-derived mammalian embryos remain high. Much less is known about the role of ooplasm in the early ontogeny of fishes, but since the embryo of most fish species is totally reliant on nutrients in the egg yolk, the influence of the ooplasm is likely to be significant. Moreover, some maternal hormones and some contaminants that are known to disrupt endocrine physiology in adult fish are transferred from maternal blood to the oocytes during the maturation of fish ovaries; this uncontrolled delivery of biologically active hormones and endocrine disrupting compounds may have significant consequences for the early development of the embryo either by acting on hormone receptors, or by inhibiting the clearance of native hormones. We propose to examine the impact of the stress hormone, cortisol, on the expression of key growth-related genes during early embryogenesis; egg cortisol content varies with the maternal plasma hormone levels (and hence stress levels) of the brood-stock, but its actions on embryo development is poorly understood. In addition, we propose to examine the effect of two classes of endocrine disrupting compounds, a xenoestrogen (bisphenol A) and an activator of the hepatic aryl hydrocarbon receptor (TCDD) on the expression of the same genes. The effects of these factors on the acute stress-response, and the expression of growth related genes will be examined in embryos and early stage juveniles.
脊椎动物卵母细胞的细胞质在调节或修饰由该卵母细胞发育的胚胎的早期基因表达中起关键作用,并且可以影响胚胎后阶段的表型。 尽管对哺乳动物进行了数十年的深入研究,导致了生殖生物技术的发展,如体外受精(IVF),但对卵质因素的表观遗传影响仍然知之甚少; IVF衍生的哺乳动物胚胎的胚胎畸形率仍然很高。 关于卵质在鱼类早期个体发育中的作用知之甚少,但由于大多数鱼类的胚胎完全依赖于蛋黄中的营养物质,卵质的影响可能是显着的。 此外,一些母体激素和一些污染物是已知的破坏内分泌生理学在成鱼从母体血液中转移到卵母细胞在成熟的鱼卵巢;这种不受控制的交付的生物活性激素和内分泌干扰化合物可能有显着的后果,胚胎的早期发育,无论是通过作用于激素受体,或通过抑制清除天然激素。 我们建议研究应激激素,皮质醇,在早期胚胎发育过程中的关键生长相关基因的表达的影响;鸡蛋皮质醇含量随母体血浆激素水平(因此应力水平)的亲鱼,但其对胚胎发育的作用知之甚少。 此外,我们建议检查两类内分泌干扰化合物,异雌激素(双酚A)和激活剂的肝芳烃受体(TCDD)对相同基因的表达的影响。这些因素对急性应激反应的影响,以及生长相关基因的表达将在胚胎和早期的青少年进行检查。

项目成果

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Leatherland, John其他文献

Leatherland, John的其他文献

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{{ truncateString('Leatherland, John', 18)}}的其他基金

Stress- and xenobiotic-induced changes in early fish embryo gene expression patterns as biomarkers of development and growth in juveniles
应激和外源性诱导的早期鱼胚胎基因表达模式的变化作为幼鱼发育和生长的生物标志物
  • 批准号:
    6974-2008
  • 财政年份:
    2012
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Stress- and xenobiotic-induced changes in early fish embryo gene expression patterns as biomarkers of development and growth in juveniles
应激和外源性诱导的早期鱼胚胎基因表达模式的变化作为幼鱼发育和生长的生物标志物
  • 批准号:
    6974-2008
  • 财政年份:
    2011
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Stress- and xenobiotic-induced changes in early fish embryo gene expression patterns as biomarkers of development and growth in juveniles
应激和外源性诱导的早期鱼胚胎基因表达模式的变化作为幼鱼发育和生长的生物标志物
  • 批准号:
    6974-2008
  • 财政年份:
    2010
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Stress- and xenobiotic-induced changes in early fish embryo gene expression patterns as biomarkers of development and growth in juveniles
应激和外源性诱导的早期鱼胚胎基因表达模式的变化作为幼鱼发育和生长的生物标志物
  • 批准号:
    6974-2008
  • 财政年份:
    2009
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Yolk hormones and early development and growth of fish embryos: basic biology and toxicological considerations
卵黄激素与鱼胚胎的早期发育和生长:基本生物学和毒理学考虑
  • 批准号:
    6974-2003
  • 财政年份:
    2007
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Yolk hormones and early development and growth of fish embryos: basic biology and toxicological considerations
卵黄激素与鱼胚胎的早期发育和生长:基本生物学和毒理学考虑
  • 批准号:
    6974-2003
  • 财政年份:
    2006
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Leica motorized cryostat
徕卡电动低温恒温器
  • 批准号:
    330126-2006
  • 财政年份:
    2005
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Research Tools and Instruments - Category 1 (<$150,000)
Yolk hormones and early development and growth of fish embryos: basic biology and toxicological considerations
卵黄激素与鱼胚胎的早期发育和生长:基本生物学和毒理学考虑
  • 批准号:
    6974-2003
  • 财政年份:
    2005
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Yolk hormones and early development and growth of fish embryos: basic biology and toxicological considerations
卵黄激素与鱼胚胎的早期发育和生长:基本生物学和毒理学考虑
  • 批准号:
    6974-2003
  • 财政年份:
    2004
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual
Yolk hormones and early development and growth of fish embryos: basic biology and toxicological considerations
卵黄激素与鱼胚胎的早期发育和生长:基本生物学和毒理学考虑
  • 批准号:
    6974-2003
  • 财政年份:
    2003
  • 资助金额:
    $ 3.13万
  • 项目类别:
    Discovery Grants Program - Individual

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