Investigation of the mechanisms involved in the assembly of COPI-coated vesicles by cryo-electron microscopy

通过冷冻电子显微镜研究 COPI 包被的囊泡组装机制

• 批准号: 355873-2008
• 负责人: Rouiller, Isabelle
• 金额: $ 2.19万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=402745
• 关键词: Investigation; mechanisms; involved; assembly; COPI

In addition to allow life forms to exist, cellular membranes organize the interior of the eukaryotic cell into biochemically distinct compartments in which specific cellular functions are performed. The transport of proteins (cargo) between discontinuous sub-cellular compartments relies on the assembly and disassembly of multilayered coat protein complexes (CPCs) onto cellular membrane. To-date, three major types of vesicles have been described: clathrin-, COPI (coat protein complex I)- and COPII (coat protein complex II)-coated vesicles. Clathrin-coated vesicles bud from the plasma membrane, trans-Golgi network (TGN) and endosomes. COPI-coated vesicles traffic primarily from the Golgi to the endoplasmic reticulum (ER) and between Golgi cisternae. COPII-coated vesicles traffic from the ER to the Golgi. The structures of clathrin and COPII coated vesicles have recently been published. Although these vesicles appear to share some common mechanical designs, there are significant differences in the way the vesicles are assembled.

除了允许生命形式存在外，细胞膜还将真核细胞的内部组织成生物化学上不同的隔间，在这些隔间中执行特定的细胞功能。蛋白质（货物）在不连续的亚细胞间的运输依赖于细胞膜上多层外壳蛋白复合物（CPCs）的组装和拆卸。迄今为止，已经描述了三种主要类型的囊泡：网格蛋白-，COPI（外壳蛋白复合体I）-和COPII（外壳蛋白复合体II）-包被囊泡。网格蛋白包被的囊泡从质膜、反式高尔基网络（TGN）和核内体中萌发。copi包被的囊泡主要从高尔基体到内质网（ER）和高尔基池之间运输。copii包被囊泡从急诊室到高尔基体的交通。网格蛋白和COPII包被囊泡的结构最近已经发表。尽管这些囊泡似乎有一些共同的机械设计，但在囊泡的组装方式上存在显著差异。