Investigation of the mechanisms involved in the assembly of COPI-coated vesicles by cryo-electron microscopy

通过冷冻电子显微镜研究 COPI 包被的囊泡组装机制

• 批准号: 355873-2008
• 负责人: Rouiller, Isabelle
• 金额: $ 2.19万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=402745
• 关键词: Investigation; mechanisms; involved; assembly; COPI

In addition to allow life forms to exist, cellular membranes organize the interior of the eukaryotic cell into biochemically distinct compartments in which specific cellular functions are performed. The transport of proteins (cargo) between discontinuous sub-cellular compartments relies on the assembly and disassembly of multilayered coat protein complexes (CPCs) onto cellular membrane. To-date, three major types of vesicles have been described: clathrin-, COPI (coat protein complex I)- and COPII (coat protein complex II)-coated vesicles. Clathrin-coated vesicles bud from the plasma membrane, trans-Golgi network (TGN) and endosomes. COPI-coated vesicles traffic primarily from the Golgi to the endoplasmic reticulum (ER) and between Golgi cisternae. COPII-coated vesicles traffic from the ER to the Golgi. The structures of clathrin and COPII coated vesicles have recently been published. Although these vesicles appear to share some common mechanical designs, there are significant differences in the way the vesicles are assembled.

除了允许生命形式存在外，细胞膜还将真核细胞的内部组织到生化不同的区室中，其中进行了特定的细胞功能。不连续的亚细胞室之间蛋白质（货物）的转运取决于多层外套蛋白质复合物（CPC）的组装和拆卸到细胞膜。迄今为止，已经描述了三种主要类型的囊泡：网格蛋白，COPI（外套蛋白质复合物I）和copii（外套蛋白质复合物II）涂成囊泡。网状蛋白包被的囊泡来自质膜，跨加利基网络（TGN）和内体。 copi涂层的囊泡主要从高尔基体到内质网（ER）以及高尔基硫酸盐之间。 copii涂层的囊泡从急诊室到高尔基。网格蛋白和copii涂层囊泡的结构最近发表了。尽管这些囊泡似乎共享一些常见的机械设计，但在组装囊泡的方式上存在显着差异。