Precommercialization of enviropig(TM) biotechnology Pt2

enviropig(TM) 生物技术 Pt2 预商业化

• 批准号: 369186-2008
• 负责人: Forsberg, Cecil
• 金额: $ 2.9万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=409918
• 关键词: Precommercialization; enviropig; TM; biotechnology; Pt2

The swine industry in Canada is a multibillion-dollar business with approximately 14 million pigs on farms. The three major nutrients for their growth and development are carbohydrate, protein and phosphorus, and these are provided by cereal grains and plant protein supplements. Phosphorus, the third major nutrient, is present in these feed components largely in the form of phytic acid, which is not digestible by the pig. To solve the inability to digest phytate we have genetically enhanced a line of Yorkshire pigs trademarked "EnviropigTM" to secrete the enzyme phytase in the salivary that enables digestion of phytate in the stomach with the release of phosphate which is readily absorbed in the small intestine. Since supplementation of the diet with available phosphate is unnecessary, there is greater utilization of the phosphorus in the cereals with a corresponding reduction of phosphorus in the manure by as much as 60%. Therefore, these pigs cost marginally less to feed, and they produce manure that has less pollution potential. We have done extensive testing which documents that the pigs grow normally, are identical to conventional pigs in all other ways other than salivary phytase production, and that the non-allergenic phytase protein is present only in the salivary glands and some secretory glands of the upper respiratory tract. The objective of this research is to complete the food safety assessment and stability testing of phenotypic expression, and to assess the effect of manure from the Enviropigs as a fertilizer for plant growth. The final objective is to develop a website containing food and environmental safety information, and efficacy data that will provide an inexpensive, but efficient conduit for global access by regulators, investors, scientists, producers, and consumers, and their various organizations. We will conduct the animal studies and assemble the information for the website, while the plant research will be completed through collaboration with soil scientists, and the database assembled with assistance of information management experts.

加拿大的养猪业是一项价值数十亿美元的业务，农场上约有1400万头猪。它们生长发育的三种主要营养素是碳水化合物、蛋白质和磷，这些营养素由谷物和植物蛋白补充剂提供。磷是第三种主要营养素，主要以植酸的形式存在于这些饲料成分中，植酸不能被猪消化。为了解决不能消化植酸盐的问题，我们对一系列注册商标为“EnviropigTM”的约克郡猪进行了基因增强，使其在唾液中分泌植酸酶，从而能够在胃中消化植酸盐，并释放易于在小肠中吸收的磷酸盐。由于不需要在饮食中补充可利用的磷酸盐，因此谷物中的磷得到了更大的利用，相应地粪便中的磷减少了60%。因此，这些猪的饲养成本略低，而且它们产生的粪便污染可能性较小。我们已经进行了广泛的测试，证明猪生长正常，除了唾液植酸酶的产生之外，在所有其他方面与传统猪相同，并且非过敏性植酸酶蛋白仅存在于唾液腺和上呼吸道的一些分泌腺中。本研究的目的是完成食品安全性评估和表型表达的稳定性测试，并评估环境猪粪便作为植物生长肥料的效果。最终目标是开发一个包含食品和环境安全信息以及功效数据的网站，为监管机构、投资者、科学家、生产者和消费者及其各种组织提供一个廉价但有效的渠道。我们将进行动物研究，并为网站收集信息，而植物研究将通过与土壤科学家合作完成，数据库将在信息管理专家的协助下收集。