Swinging-bucket ultracentrifuge rotor

我正在申请资金，以购买一个摆动桶，钛转子，用于超高速离心机，以支持在UNB Fredericton生物系的研究。超离心机是任何分子生物学/生物化学实验室所需的基本实验设备，其功能是基于样品以非常高的速度绕固定轴旋转。这种加速度产生向心力，用于根据密度和/或大小对细胞悬浮液进行分级。摇斗式转子具有较长的路径长度，非常适合使用密度梯度离心法分离细胞成分和蛋白质复合体。我的研究利用这项技术来检测与所有光合作用真核生物中发现的捕光复合体(LHC)相关的蛋白质的细胞位置和生化性质，LHC可能在保护光合作用装置免受过量光损害方面起到作用。这些蛋白质预计会结合色素，并可能在胁迫期间起到消散光能或以其他方式保护光合作用装置的作用，因为在胁迫期间，过量的光可能是破坏性的。剖析这一LHC相关蛋白家族的作用对于预测微藻耐受压力增加的能力并最终有助于了解微藻如何调节采光和能量产生具有重要意义。

I am requesting funds to purchase a swinging bucket, titanium rotor that is used in ultra-high speed centrifugation to support research in the Biology Department at UNB Fredericton. The ultracentrifuge is basic piece of laboratory equipment required in any molecular biology/biochemistry laboratory and its function is based on rotation of samples around a fixed axis at very high speeds. This acceleration creates centripetal forces that are used to fractionate cellular suspensions on the basis of density and/or size. Swinging-bucket rotors have a long path length that is ideal for fractionating cellular components and protein complexes using density gradient centrifugation. My research utilizes this technique to examine the cellular location and biochemical properties of proteins that are related to the light harvesting complexes (LHC) found in all photosynthetic eukaryotes, which may have a role in protecting the photosynthetic apparatus from damage due to excess light. These proteins are predicted to bind pigments and may have a role in dissipating light energy or otherwise protecting the photosynthetic apparatus during periods of stress when excess light can be destructive. Dissecting the role of this family of LHC-related proteins is important to predict the capacity of microalgae to tolerate increased stress conditions and ultimately contribute to the knowledge of how light harvesting and energy generation are regulated in microalgae.