Regulation & function of proteins at prophase of meiosis

规定

• 批准号: 1901-2008
• 负责人: Moens, Peter
• 金额: $ 3.64万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=419506
• 关键词: Regulation; function; proteins; prophase; meiosis

Our research contributions over the last decade have established us among the authorities in the field of germ cell development, specifically in functional and structural aspects of chromosomes at prophase of meiosis. Unlike somatic (body) cells that can go through repeated cell division and growth cycles in the body or in tissue culture, germ cells have a single development pathway - they do not cycle and there is no efficient method available for their culture. Fortunately, it has been discovered that germ cells will develop rapidly - hours instead of days - when cultured in the presence of the phosphatase inhibitor, okadaic acid. With this technique, our laboratory and the laboratory of M.A. Handel have embarked on the analysis of factors that affect germ cell development. We investigate genetic mutations that affect meiosis as well as the effect of toxic substances and the experimental repression of proteins required for meiosis. The meiotic systems that we study are associated with fertilty defects and deregulation of cell proliferation (cancer). In addition, we are enlisted as consultants/research collaborators by two or three laboratories each year.

在过去的十年里，我们的研究贡献确立了我们在生殖细胞发育领域的权威地位，特别是在减数分裂前期染色体的功能和结构方面。与在体内或组织培养中可以经历反复细胞分裂和生长周期的体细胞(身体)细胞不同，生殖细胞只有一条发育途径-它们不会循环，也没有有效的方法进行培养。幸运的是，人们已经发现，在磷酸酶抑制剂冈田酸存在的情况下，生殖细胞将迅速发育--几小时而不是几天。利用这项技术，我们的实验室和M.A.Handel的实验室已经开始分析影响生殖细胞发育的因素。我们研究影响减数分裂的基因突变，以及有毒物质的影响和对减数分裂所需蛋白质的实验性抑制。我们研究的减数分裂系统与生育缺陷和细胞增殖的放松调控(癌症)有关。此外，我们每年被两三个实验室招募为顾问/研究合作者。