Functional genomics of follicular growth and ovulation

卵泡生长和排卵的功能基因组学

• 批准号: 104199-2008
• 负责人: Lussier, Jacques
• 金额: $ 3.06万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=421647
• 关键词: Functional; genomics; follicular; growth; ovulation

This research program aims at the identification and the study of molecular determinants that control the development of the ovarian follicle and oocyte, in relation to follicular selection/dominance and ovulation. The cow is used as the working model since it mimics the physiological pattern of follicular development observed in women, and represents an in vivo model of economic importance. Molecular determinants identified through the application of cDNA subtractive hybridization has allows the establishment of a gene index for granulosa cells at different follicular stages, by comparing non-selected to dominant follicles, and dominant to ovulatory follicles. The expression pattern of genes (known and novel cDNAs) identified, will be investigated by RT-PCR at different stages of follicular development and ovulation. The novel cDNAs identified, will be characterized by cloning and sequencing of their full-length cDNA. The study of the spatio-temporal expression pattern of genes in relation to follicular growth and ovulation will increase our understanding and control of this physiological process: to identify novel genes involved in cellular proliferation and cell signaling (extra and intra cellular) that impact on tissue remodeling, healing, and inflammatory processes; to increase the production of competent oocytes used for in vitro fertilization, nuclear transfer and transgenesis; to increase the efficacy of treatments aiming at follicle synchronization and ovarian super-stimulation programs in domesticated animals of economic importance, and in fertility and contraception programs in humans; and to identify candidate genes associated with fertility that can be used as molecular markers for genetic assisted selection in breeding programs of industries involved in commercializing and distributing male and female gametes.

该研究计划旨在识别和研究控制卵泡和卵母细胞发育与卵泡选择/优势和排卵相关的分子决定因素。牛被用作工作模型，因为它模仿了在女性中观察到的卵泡发育的生理模式，并且代表了具有经济重要性的体内模型。通过应用 cDNA 消减杂交鉴定的分子决定簇，通过比较非选择卵泡与优势卵泡以及优势卵泡与排卵卵泡，可以建立不同卵泡阶段颗粒细胞的基因指数。所鉴定的基因（已知和新的 cDNA）的表达模式将通过 RT-PCR 在卵泡发育和排卵的不同阶段进行研究。鉴定出的新型 cDNA 将通过其全长 cDNA 的克隆和测序来表征。对与卵泡生长和排卵相关的基因的时空表达模式的研究将增加我们对这一生理过程的理解和控制：识别参与细胞增殖和细胞信号传导（细胞外和细胞内）的新基因，这些基因影响组织重塑、愈合和炎症过程；增加用于体外受精、核移植和转基因的活性卵母细胞的产量；提高针对具有经济重要性的家养动物的卵泡同步和卵巢超刺激计划以及人类生育和避孕计划的治疗效果；并鉴定与生育力相关的候选基因，这些基因可用作涉及商业化和分配雄性和雌性配子的行业育种计划中遗传辅助选择的分子标记。