DDB1 complexes in arabidopsis visible and UV light response

拟南芥可见光和紫外光响应中的 DDB1 复合物

• 批准号: 261810-2008
• 负责人: Schroeder, Dana
• 金额: $ 2.49万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=426816
• 关键词: DDB1; complexes; arabidopsis; visible; UV

Plants are very sensitive to light. When grown in the dark, such as under a rock or board, plant seedlings are tall, thin, pale, and keep their leaves closed. Bean sprouts are an example of this type of growth. When plants grow in the light though they are short and green with leaves open to absorb the sunlight. Our studies in the mustard relative Arabidopsis have centred around a mutant, det1, that is defective in this process - it looks like a light-grown plant even when grown in the dark. This suggests that DET1 is normally an important part of how the plant responds to light. We are trying to understand how DET1 performs this role. We have found that the DET1 protein interacts with a protein called Damaged DNA-Binding protein 1 (DDB1). DDB1 was originally identified in humans, where it is involved in the repair of UV-damaged DNA. DDB1 activity is defective in some human xeroderma pigmentosa patients. These people are at risk for skin cancer because they are unable to repair DNA damage caused by UV light. Our result is very exciting because it suggests that there may be a common mechanism of responding to "good" light, which makes plants grow, and "bad" UV light, which is damaging to all life forms. We hope by studying DET1 and DDB1 to help design crop plants with both optimal light response and UV protective capacity.

植物对光非常敏感。当在黑暗中生长时，如在岩石或木板下，植物幼苗高，瘦，苍白，并保持叶子关闭。 豆芽就是这种生长方式的一个例子。 当植物在光照下生长时，虽然它们是短的和绿色的，叶子张开来吸收阳光。 我们在芥菜类拟南芥中的研究集中在一个突变体det 1上，它在这个过程中有缺陷-即使在黑暗中生长，它看起来也像一个光生长的植物。 这表明DET 1通常是植物对光反应的重要组成部分。 我们正在试图了解DET 1如何发挥这一作用。 我们发现DET 1蛋白与一种称为受损DNA结合蛋白1（DDB 1）的蛋白相互作用。 DDB 1最初是在人类中发现的，它参与修复紫外线损伤的DNA。 DDB 1活性在一些人类着色性干皮病患者中是缺陷的。 这些人有患皮肤癌的风险，因为他们无法修复紫外线造成的DNA损伤。 我们的结果非常令人兴奋，因为它表明可能存在一种共同的机制来响应“好”光，使植物生长，以及“坏”紫外线，这对所有生命形式都是有害的。 我们希望通过研究DET 1和DDB 1来帮助设计具有最佳光响应和紫外线防护能力的作物。