High resolution multisensor respirometer

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With this grant we will purchase an Oroboros Oxygraph 2K that measures dissolved oxygen and ions. It will allow us to accurately measure rates of oxygen consumption of cells and mitochondria. Every animal cell contains mitochondria which consume oxygen while converting the products of ingested food into usable energy. Changes in the way mitochondria consume oxygen are important to many natural and pathological phenomena. For example, in hibernation a mammal's rate of oxygen consumption decreases by up to 90% as its body temperature falls close to freezing. Liver mitochondrial metabolism is decreased by 70% in hibernation and, using the Oxygraph, we will investigate how this occurs. These findings may improve treatment for traumatic injury and the viability of organs used for transplant.